不同剂量硒蛋白粉对大鼠血常规指标和血液生化指标的影响  

Effects of Different Doses of Selenium Protein Powder on Hematological Variables and Blood Biochemistry Indices of Rats

作　　者：王昕 王岩[2] 秦顺义[1] 王宏祥[1] 王景申 

机构地区：[1]天津农学院动物科学系,天津300384 [2]天津医科大学实验动物科学部,天津300070 [3]天津市西奈克生物技术有限公司,天津301706

出　　处：《中国畜牧兽医》2014年第7期148-152,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目"富硒乳酸菌拮抗黄曲霉毒素对肉仔鸡毒性作用的效果及分子机理研究"(31101867)

摘　　要：本试验旨在研究不同剂量硒蛋白粉对大鼠血常规指标及血液生化指标的影响。选择平均体重为70g的健康清洁级SD大鼠80只,随机分为4组,每组2个重复,每个重复10只,雌、雄各半。对照组饲喂基础日粮,低、中和高剂量组分别饲喂添加3.5、7和10.5mg/kg硒蛋白粉的日粮(其含量以硒计),试验期30d。结果表明,低、中和高剂量组大鼠血常规指标与对照组相比差异均不显著(P>0.05);低、中和高剂量组大鼠血清中血糖、总蛋白含量与对照组相比差异均不显著(P>0.05),低剂量组大鼠血清中甘油三酯、低密度脂蛋白含量均显著低于对照组(P<0.05);中剂量组大鼠血清中白蛋白/球蛋白及甘油三酯、低密度脂蛋白含量均显著低于对照组(P<0.05),而谷丙转氨酶和谷草转氨酶水平均显著高于对照组(P<0.05);高剂量组大鼠血清中白蛋白、白蛋白/球蛋白、甘油三酯、低密度脂蛋白含量均显著低于对照组(P<0.05),而球蛋白、谷丙转氨酶、谷草转氨酶、尿素氮、肌酐、高密度脂蛋白含量均显著升高(P<0.05)。综上所述,不同剂量硒蛋白粉对大鼠血常规指标无不良影响,低剂量硒蛋白粉除具有降低大鼠血脂作用外对大鼠其他血液生化指标均无不良影响,中、高剂量硒蛋白粉对大鼠的部分血液生化指标有一定影响。This experiment was conducted to study the effects of different doses of selenium protein powder on hematologi-cal variables and blood biochemistry indices of rats. Eighty healthy SD rats, average body weight was 70 g, were randomly as-signed into four groups,each group had two replications,every replication had 10 rats （male and female in half）. Rats in controlgroup were fed with basal diet,low-dose group,middle-dose group and high-dose group were fed with the diet which had 3.5,7and 10.5 mg/kg selenium protein powder （calculated as the contains of selenium） on it,respectively. The experiment lasted for30 days. The results showed that the hematological variables of low-dose group, middle-dose group and high-dose group werenot significantly different compared with the control group （P〈0.05）, and there were no significant differences in the serumGLU and TP levels among all groups （P〈0.05） ,the serum TG,LDL levels in the low-dose group were significantly lower thanthat in the control group （P〈0.05） ,the serum A/G,TG,CHO and LDL levels in the middle-dose group were significantly low-er than that in the control group （P〈0.05）, the serum ALT, AST levels in the middledose group were significantly higherthan that in the control group （P〈0.05）,the serum ALB, A/G,TG and LDL levels in the high-dose group were significantlylower than that in the control group （P〈0. 05） ,while the serum GLB, ALT, AST, BUN, creatinine and HDL levels in thehigh-dose group were significantly increased （P〈0.05）. The results indicated that different doses of selenium protein powderhad no significant effect on hematological variables in rats,and low-dose selenium protein powder had no significant effect onblood biochemistry indices except for the indices of lipid metabolism, middle-doses and high-doses selenium protein powder hadsome influence on blood biochemistry indices in rats.

关 键 词：硒 大鼠 血常规 血液生化指标 

分 类 号：S816[农业科学—饲料科学]