2种不同检测模式下的林可霉素胶体金试纸条的比较研究  

Study on Two Patterns Comparison of Gold Immunochromatography Test Strip for Rapid Detection of Lincomycin

作　　者：周洁[1] 徐飞[1] 吴超[1] 刘文丽[1] 丁双阳[1,2] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]国家兽药残留基准试验室,北京100193

出　　处：《中国畜牧兽医》2014年第7期159-163,共5页China Animal Husbandry & Veterinary Medicine

基　　金：公益性行业(农业)科研专项经费项目(201203069-2);奶牛产业体系北京市奶牛创新团队资金

摘　　要：本试验旨在研究检测牛奶中林可霉素(lincomycin,LIN)胶体金免疫层析试纸条。根据直接竞争抑制和间接竞争抑制2种模式,通过对金标包被原(抗体)量、pH、包被抗体(抗原)浓度、牛奶前处理等试验的优化,制备出2种满足实际检测需要的胶体金试纸条;二者的检测限分别为30和10ppb。在实际检测中,间接法在灵敏度、稳定性和检测时间等方面都优于直接法。The objective of this study was to develop gold immunochromatography test strips to detect lincomycin in milk.Based on two patterns of direct competitive inhibition and indirect competitive inhibition, we optimized concentration of immunogold labeling BSA-LIN （IgG）, pH, concentration of coating IgG （BSA-LIN）, pretreatment of milk and so on. The resultsshowed that we got two strips that fulfil practical requirement. The LOD of direct and indirect were 30 and 10 ppb. Comparingtwo methods, indirect method was better than direct in sensitivity, stability and testing time.

关 键 词：林可霉素 胶体金免疫层析 直接竞争法 间接竞争法 牛奶 

分 类 号：S859.796[农业科学—临床兽医学]