化学发光免疫测定法检测HBV、抗HCV、抗HIV和TP-Ab结果分析  

The results analysis of detection HBV,anti-HCV,anti-HIV and TP-Ab by chemiluminesce immunoassay

作　　者：巫文勋 程利 梁可 刘萍 王长奇 

机构地区：[1]湘雅萍矿合作医院检验科,江西萍乡337000 [2]博奥赛斯(天津)生物科技有限公司,300162

出　　处：《重庆医学》2014年第20期2626-2628,2631,共4页Chongqing medicine

摘　　要：目的：对患者输血前或手术前的血液传染性疾病标志物进行检测分析。方法采用化学发光免疫测定（CLIA）法和ELISA法对湘雅萍矿合作医院3542例血清标本的HBsAg、抗HBs、HBeAg、抗HBe、抗HBc、抗丙型肝炎病毒（抗HCV）、抗人类免疫缺陷病毒（抗 HIV）、梅毒螺旋体抗体（TP-Ab）进行了检测。结果利用CLIA法检测3542例标本中乙型肝炎阳性704例，检出率为19．8％；3032例标本中丙型肝炎阳性52例，检出率为1．72％；3049例标本中抗 H IV阳性3例，检出率为0．09％；3153例标本中TP-Ab阳性200例，检出率为6．34％；不同性别乙型肝炎病毒（HBV）、抗 HCV、抗 HIV和 TP-Ab感染率有所不同，其中HBV、抗HCV和TP-Ab 3项，男性检出率明显高于女性（P＜0．05）；不同年龄段 HBV、抗 HCV、抗 HIV和TP-Ab感染率有所不同，HBV和抗 HCV以21～40岁检出率最高，分别为31．9％和2．33％；TP-Ab检出率随着年龄增大有增加的趋势，60岁以上检出率最高，达7．55％；经上述两种方法检测，HBsAg测值不一致的有51例，占1．44％，抗 HCV测值不一致的有1例，占0．03％，TP-Ab测值不一致的有3例，占0．1％，抗 HIV测值完全符合，均有3例阳性，不一致的标本经第三方试剂验证，结果CLIA法测值较为准确。结论 CLIA法是一种高灵敏度的检测方法，可有效地避免阳性标本漏检，应用CLIA法在输血或手术前对患者进行血液传染病性标志物检测具有重要意义。Objective To detection analysis of blood infectious disease markers in patients before blood transfusion or before surgery .Methods HBsAg ,anti-HBs ,HBeAg ,anti-HBe ,anti-HBc ,anti-HCV ,anti-HIV ,TP-Ab were detected for 3 542 cases of se-rum using chemiluminescence immunoassay（CLIA） and ELISA .Results For HBsAg ,there were 704 cases of positive among 3 542 cases ,the proportion was 19 .8% ;For anti-HCV ,there were 52 cases of positive among 3 032 cases ,the proportion was 1 .72% ;For anti-HIV ,there were 3 cases of positive among 3 049 cases ,the proportion was 0 .09% ;For TP-Ab ,there were 200 cases of positive among 3 153 cases ,the proportion was 6 .34% .The infection rate was different in different gender of HBV ,anti HCV ,anti HIV and TP-Ab .But for HBV ,anti-HCV and TP-Ab ,the detection rate of male was higher than female（P〈0 .05）;The infection rate was different in different age of HBV ,anti HCV ,anti HIV and TP-Ab different age .The detection rate of HBV and Anti-HCV in 21-40 years old was the highest ,They were 31 .9% and 2 .33% ;The detection rate of TP-Ab increases with age increased ,more than 60 years old was the highest ,up to 7 .55% .Detection of the above two methods ,for HBsAg ,there were 51 cases did not match ,the proportion was 1 .44% ;for anti-HCV ,there were 1 case did not match ,the proportion was 0 .03% ;for Tp-Ab ,there were 3 cases did not match ,the proportion was 0 .1% ;for anti-HIV ,CLIA agrees well with ELISA ,there were 3 cases of positive .The samples that did not match were detected by the third party verification of the reagent .The result displayed that CLIA was more accurate . Conclusion CLIA is a high-sensitivity detection method which could effectively avoid positive specimens missed ,detection of infec-tious disease markers in patients before transfusion or before surgery by CLIA had important significance .

关 键 词：获得性免疫缺陷综合征 梅毒 化学发光免疫分析系统 术前检查 乙型肝炎病毒 

分 类 号：R446.6[医药卫生—诊断学]