阿霉素诱导肝癌QGY-7701细胞凋亡的研究  

The study of apoptosis induced by doxorubicin hydrochloride in QGC-7701 cell

作　　者：夏艳[1] 邵淑丽[1] 张希锐[1] 张伟伟[1] 李凤英[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2014年第4期60-63,共4页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省自然科学基金项目(C200624;C201241);黑龙江省教育厅科学技术项目(11511447;12511611)

摘　　要：观察阿霉素对人肝癌细胞株QGY-7701的凋亡诱导作用。实验用阿霉素处理QGY-7701细胞后,用台盼蓝拒染法检测细胞增殖活性;光镜、荧光显微镜、透射电镜观察细胞凋亡形态;DNA琼脂糖凝胶电泳技术观察分析细胞凋亡情况;流式细胞仪检测细胞凋亡率的变化。结果显示,阿霉素处理QGY-7701细胞后,出现了明显的凋亡现象,而且随着药物浓度的增加,细胞凋亡的趋势越明显。分析结果可知,阿霉素作用QGY-7701细胞48 h的IC50值为4.9μg/mL。流式细胞仪检测出阿霉素处理QGY-7701细胞的凋亡率逐渐上升。综上所述,阿霉素可以诱导QGY-7701细胞出现凋亡的现象。To evaluate the anti-tumor effect of ADM on the human hepatocarcinoma cells line QGY-7701. cell viability and proliferration assay was evaluated by trypan blue-rejected staining method. The apoptosis of QGY-7701 cells induced by different concentrations of ADM,to the human hepatocarcinoma cells line QGY-7701 was analyzed by visible light,fluorescence and transmission electron microscope（TEM）,agrose gel electrophoresis of DNA;Distribution of cell cycle and apoptosis was analyzed using flow cytometry;Results shows the typical apoptosic morphological changes showed in apoptosis of QGY-7701 cells induced by ADM. Different concentrations of ADM were to human hepatocarcinoma cancer cells line QGY-7701,the IC50 of ADM was 4.9μg/mL . The ADM reduced the growth of the human hepatocarcinoma cells line QGY-7701 and increasing apoptotic cell death.

关 键 词：阿霉素 QGY-7701 细胞凋亡 

分 类 号：Q291[生物学—细胞生物学]