多重耐药淋病奈瑟菌耐药基因研究  

Study on Drug-Resistance Genes of Neisseria Gonorrhoeae with Multi-drug Resistance

作　　者：侯玲俐[1] 杨宏伟[2] 吕军[2] 张吉才[2] 曹峰[2] 朱名安[2] 

机构地区：[1]湖北医药学院附属太和医院输血科,湖北十堰442000 [2]湖北医药学院附属太和医院检验部,湖北十堰442000

出　　处：《湖北医药学院学报》2014年第3期240-243,共4页Journal of Hubei University of Medicine

基　　金：十堰市科学技术研究与开发项目(ZD2011007);湖北医药学院研究生启动金(2011QDJ09)

摘　　要：目的:探讨淋病奈瑟菌多重耐药表型与耐药基因型之间的关系。方法:应用K-B法检测临床分离到的40株淋病奈瑟菌对8种常用抗生素的敏感性。用煮沸法提取细菌的DNA,用PCR法检测随机抽取其中20株分离株的TEM、tetM、ermF和mefA基因。结果:淋病奈瑟菌的多重耐药现象明显,对氧氟沙星和环丙沙星的耐药率均高达92.5%;TEM、tetM基因的检出率均为75.0%,ermF和mefA基因未检出;本组菌株对青霉素的耐药表型与TEM基因密切相关(Kappa=0.733,P<0.01)。结论:淋病奈瑟菌的多重耐药表型与耐药基因之间密切相关。Objective To explore the relationship between multi-drug resistance of Neisseria gonorrhoeae（ N. gonorrhoeae）phenotype and resistance genes. Methods The K-B method was used to analyze the sensitivity of 40 clinical strains of N.gonorrhoeae exposured to 8 kinds of common antibiotics. The DNA of the strains was extracted with boiling method,and the resistance genes such as TEM,tetM,ermF and mefA were detected by PCR in the randomly selected 20 strains. Results The multi-drug resistance was obviously observed in N. gonorrhoeae. The resistant ratio of N. gonorrhoeae to ofloxacin and ciprofloxacin was both at about 92. 5%. The positive rate of TEM and tetM genes were both 75. 0%,but ermF and mefA genes were not detected in the 20 strains. The drug-resistance phenotype of the 20 strains to penicillin was significantly correlated with the expression of TEM gene（ Kappa = 0. 733,P〈0. 01）. Conclusion There was a close relationship between the multidrug resistance phenotype of N. gonorrhoeae and the drug-resistance genes.

关 键 词：淋病奈瑟菌 多重耐药 耐药基因 

分 类 号：R446.5[医药卫生—诊断学] R759.2[医药卫生—临床医学]