湖北海棠叶水煎剂对HSV-1病毒性结膜炎治疗作用  

Efficacy of Hubei Wingnut (Malus hapehensis) Leaf Decoction on Viral Conjunctivitis Infected with HSV-1

作　　者：李祖铭 孔丽华 余玲 王青 黄朝明 席祖莲 屈克义 李建蓉[3] 

机构地区：[1]湖北省宜昌市夷陵区妇幼保健院,宜昌443100 [2]湖北省宜昌市夷陵医院,宜昌443100 [3]华中科技大学同济医学院病原生物学系,武汉430030

出　　处：《医药导报》2014年第7期862-865,共4页Herald of Medicine

摘　　要：目的研究湖北海棠叶水煎剂对I型单纯疱疹病毒(HSV-1)所致结膜炎的治疗作用。方法采用湖北海棠叶水煎剂作为实验样品,用利巴韦林滴眼剂作对照。分别以HSV-1感染Vero细胞和家兔眼结膜,通过观察细胞病变效应判断药物抑制病毒复制的环节。对家兔眼结膜炎疾病模型进行眼结膜组织病理学检查,观察低、中、高浓度湖北海棠叶水煎剂对HSV-1兔眼结膜炎的治疗作用。结果体外实验中,湖北海棠叶水煎剂对HSV-1增殖无抑制作用。整体动物实验中,眼部给予湖北海棠叶水煎剂3,6 kg·L-1,连续给药7 d,家兔眼结膜病理性炎症反应明显减弱甚至消失,与0.9%氯化钠溶液对照组比较,差异有统计学意义(P<0.05)。中、高剂量组家兔眼结膜炎治疗有效率分别为83.3%和100.0%。结论湖北海棠叶水煎剂可减轻HSV-1所致家兔结膜炎炎性反应。Objective To investigate the therapeutic effect of the Hubei Wingnut （ Malus hapehensis ） leaf decoction （MHD） against conjunctivitis infected with human simplex virus type I （HSV-1）. Methods Malus hupehcnsis decoction was used as the active treatment and ribavirin （ RBV） eye drop was used as the positive control. Both of the Vero cells and rabbit eye conjunctiva were infected with HSV-1. The effect and mechanism of the MHD on viral replication was determined by observing the cytopathic effect （ CPE） . The efficacy of MHD at different doses on the rabbit viral conjunctivitis was examined by pathological changes of eye conjunctiva tissues. Results MHD did not inhibit the proliferation of HSV-1 in vitro. The inflammatory reactions of rabbit viral conjunctivitis caused by HSV-1 were obviously attenuated or disappeared after treatment with MHD at 6 kg·L-1 and 3 kg·L-1 for 7 consecutive days,compared with the negative control of 0. 9% NaCl. The curative rate of MHD at the middle and high doses was 83. 3% and 100. 0%, respectively. Conclusion MHD has the potential for treating eye conjunctivitis caused by HSV-1 by relieving inflammation.

关 键 词：湖北海棠叶 结膜炎 病毒性 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]