芪生胶囊中芍药苷的含量测定  

Determination of Paeoniflorin in Qisheng Capsule

作　　者：石小鹏[1] 缪珊[2] 毕琳琳[2] 李捷[2] 苗青[2] 党碧艳[2] 栗艳[1] 

机构地区：[1]第四军医大学西京医院药剂科,西安710032 [2]第四军医大学药学系天然药物学教研室,西安710032

出　　处：《医药导报》2014年第7期937-939,共3页Herald of Medicine

摘　　要：目的：建立测定芪生胶囊中芍药苷含量的方法。方法采用高效液相色谱（ HPLC）法测定芪生胶囊中芍药苷的含量,Kromasil C18色谱柱（4.6 mm×250 mm,5μm）；以甲醇-水（1585）为流动相；检测波长：230 nm；流速：1.0 mL·min-1。结果芍药苷在2.5-12.5μg·mL-1范围内线性关系良好,r=0.9999；平均加样回收率99.97%（ n=6）,RSD=0.94%。结论该方法简单,专属性强,重复性好,可有效控制芪生胶囊的质量。Objective To establish a content determination method for paeoniflorin in qisheng capsule. Methods The quantitative analysis of paeoniflorin in qisheng Capsule was carried out by high-performance liquid chromatography （ HPLC） . The chromatographic separation was achieved by using a Kromasil C18 chromatographic column （4. 6 mm×250 mm,5 μm） with a mobile phase consisting of methanol,water （1585） at a flow rate of 1. 0 mL·min-1 and 230 nm detection wavelength. Results The linear range was 2. 5-12. 5 μg·mL-1（ r =0. 999 9）. The average recovery and RSD of the method were 99. 97%and 0. 94%. Conclusion The method is accurate,specific,reproducible,which can effectively be used in quality control of paeoniflorin in qisheng capsule.

关 键 词：芪生胶囊 芍药苷 含量测定 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]