桑叶黄酮类成分的多指标均匀设计提取工艺分析  

Flavonoids extraction process analysis of Morus alba by multiple indicators uniform design

作　　者：林艳[1] 肖若媚[1] 林凤瑜[1] 

机构地区：[1]中山市人民医院,广东中山528403

出　　处：《赣南医学院学报》2014年第3期354-356,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:优选桑叶黄酮类成分提取的最佳工艺。方法:采用紫外分光光度法测定总黄酮的量,以桑叶总黄酮含量作为评价指标,使用索氏回流提取,采用U(94)均匀设计表设计试验,考察提取溶剂浓度、提取时间、料液比、颗粒度4种因素对总黄酮提取的影响。结果:桑叶中黄酮类成分的最佳提取条件为:乙醇浓度为60%,提取时间为8 h,料液比为1∶50,颗粒度为60目。结论:该提取工艺操作简便合理,结果稳定准确,重复性良好,具有可行性。Objective： To optimize the extractive condition of flavonoids from Morus alba. Methods： UV were established to determine total flavonoids,U（ 94） Uniform design form was selected as the experiments＇ schedule,the total flavonoids was used as evaluation indicator to and the extraction solvent concentration,extraction time,solid-liquid ratio and particle size was used as the 4 influencing factors. Results： The uniform design experiment show that ethanol concentration of 90%,the extration time of 8 hours,the solid-liquid ratio of 1∶ 50,and particle degree of 65 orders were the optimum extraction process. Conclusion： The method can quickly,accurately determine the optimum extraction technology of flavonoids from Morus alba.

关 键 词：桑叶 均匀设计法 提取工艺 总黄酮 含量测定 

分 类 号：R284.2[医药卫生—中药学]