马钱子碱固体脂质纳米粒包封率的测定  

Determination of entrapment efficiency of brucine solid lipid nanoparticles

作　　者：张亮[1] 管庆霞[1] 赵义军[1] 高佳翔[1] 李永吉[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2014年第3期264-265,274,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省哈尔滨市科技创新人才专项基金项目(青年科技创新人才)(No.20 11RFQXS102)

摘　　要：建立马钱子碱固体脂质纳米粒中药物包封率的测定方法.采用葡聚糖凝胶柱层析法分离马钱子碱固体脂质纳米粒胶体溶液,再用HPLC测定溶液中马钱子碱的质量浓度,计算包封率.该法测定马钱子碱固体脂质纳米粒中马钱子碱的平均包封率为68.13%,RSD值为0.59%.葡聚糖凝胶法简便,准确,可以作为马钱子碱固体脂质纳米粒的包封率测定方法.In order to establish a method for determining the entrapment efficiency of brucine solid lipid nanoparticles Sephadex gel were used to separate free brucine from solid lipid nan -oparticles,the concentration of brucine was determined by HPLC .Determination of the aver-age entrapment efficiency of Sephadex gel was 68.13%, RSD values were 0.59%.This method was convenient , accurate , and can be used to determine the entrapment efficiency of brucine solid lipid nanoparticles .

关 键 词：马钱子碱 固体脂质纳米粒 包封率 葡聚糖凝胶法 

分 类 号：R94[医药卫生—药剂学]