HPLC法测定复方磺胺对甲氧嘧啶片中磺胺对甲氧嘧啶的含量  

Determination of Sulfamethoxydiazine in Compound Sulfamethoxydiazine Tablets by HPLC

作　　者：陈锡龙[1] 

机构地区：[1]贵州省兽药饲料监察所,贵阳550003

出　　处：《中国兽药杂志》2014年第7期30-33,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了兽药复方磺胺对甲氧嘧啶片中磺胺对甲氧嘧啶含量的高效液相色谱测定法。以C18为固定相，0．1％磷酸溶液-乙腈（80∶20）为流动相，流速为1．0 mL/min，柱温40℃，检测波长为270 nm，采用外标法进行定量。磺胺对甲氧嘧啶的线性范围为5．0～100μg/mL，回归方程为Area＝33．56＆#215;Amt-12．15（n＝5），相关系数大于0．999。从高（0．25 g）、中（0．20 g）和低（0．15 g）三个添加水平的检测结果可以看出，磺胺对甲氧嘧啶的回收率为97．9％～100．6％。该方法简单，准确，精密度好，回收率高，可以用于测量复方磺胺对甲氧嘧啶片中磺胺对甲氧嘧啶的含量。An HPLC method has been developed to determine the content of sulfamethoxydiazine in compound sulfamethoxydiazine tablets, using C18 column as stationary phase, with a mobile phase of 0. 1% phosphoric solution-acetonitrile （80 ∶ 20） and wavelength of 270 nm. It was quantified by external standard method and the linear range of sulfamethoxydiazine was between 5. 0 and 100 μg/mL. And the regression equation was Area=33.56＆#215;Amt-12.15 with the correlation to be more than 0.999. The average recoveries at three different levels of 0.25 g, 0.20 g and 0.15 g ranged between 97.9% and 100.6%. This method was simple and accurate, and had good precision and high recoveries as well, could be used for determination of sulfamethoxydiazine in compound sulfamethoxydiazine tablets.

关 键 词：复方磺胺对甲氧嘧啶片 磺胺对甲氧嘧啶 高效液相色谱法 

分 类 号：S859.83[农业科学—临床兽医学]