A new cytochalasin from endophytic Phomopsis sp. IFB-E060  

作　　者：SHEN Li LUO Qian SHEN Zhi-Ping LI Ling-Yu ZHANG Xiao-Jun WEI Zhong-Qi FU Yi SONG Yong-Chun TAN Ren-Xiang 

机构地区：[1]Medical College, Yangzhou University [2]State Key Laboratory of Element Organic Chemistry, Nankai University [3]Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University

出　　处：《Chinese Journal of Natural Medicines》2014年第7期512-516,共5页中国天然药物（英文版）

基　　金：supported by the Natural Science Foundation of China(Nos.81121062,30701045,21132004&21072092);the Natural Science Foundation of Jiangsu Province(No.BK2009010);the Social Development Project of Yangzhou(No.YZ2011097)

摘　　要：AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses(UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS: Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J(1), cytochalasin H(2),(22E, 24S)-cerevisterol(3), ergosterol(4), and nicotinic acid(5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL-1 and 2 had an IC50 value of 15.0 μg·mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL-1. CONCLUSION: 18-Methoxy cytochalasin J(1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.AIM： To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD： Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses （UV, CD, IR, MS, 1D, and 2D NMR）. The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS： Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J （1）, cytochalasin H （2）, （22E, 24S）-cerevisterol （3）, ergosterol （4）, and nicotinic acid （5）. Compound 1 had an inhibition rate of 24.4% at 10 μg.mL-1 and 2 had an ICs0 value of 15.0 μg.mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg.mL-1. CONCLUSION： 18-Methoxy cytochalasin J （1）, produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.

关 键 词：Phomopsis sp. Sphaeropsidaceae Vatica mangachapoi ENDOPHYTE CYTOCHALASIN 

分 类 号：R284.2[医药卫生—中药学]