异丙酚、人参皂苷Rg-1、蛋白磷酸酯酶-2A和氯化锂对大鼠电休克后学习记忆及海马内谷氨酸含量的影响  

Effects of Propofol,Ginsenoside Rg-1,Protein Phosphatae-2A,and Lithium on the Learning and Memory in Rats and the Content of Glutamic Acid in Hippocampus after the Electmconrulsive Therapy

作　　者：刘超[1] 张雪宁[1] 刘东[2] 闵苏[3] 

机构地区：[1]天津医科大学第二医院放射科,天津300211 [2]天津医科大学第二医院神经外科,天津300211 [3]重庆医科大学附属第一医院麻醉科,重庆400016

出　　处：《中国医学科学院学报》2014年第3期234-240,共7页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30972831);国家博士后科学基金(2013M530880)~~

摘　　要：目的观察异丙酚、人参皂苷Rg-1、蛋白磷酸酯酶-2A(PP-2A)和氯化锂对电休克(ECT)后嗅球切除抑郁模型大鼠学习记忆和海马内谷氨酸(Glu)浓度的影响。方法按随机单位组设2个干预因素,即ECT干预(2水平:无处置、施行1疗程ECT)和药物干预(5水平:海马CA1区分别微量注射生理盐水、异丙酚、人参皂苷Rg-1、PP-2A、氯化锂,20 g/L)的所有组合(2×5析因设计)。全部ECT处置结束24 h内开始Morris水迷宫检测,高效液相色谱法检测Glu在海马内的浓度。结果单独使用异丙酚或ECT均可造成学习记忆障碍,而二者联合使用则会缓解ECT后的学习记忆障碍;人参皂苷Rg-1、PP-2A和氯化锂对学习记忆无明显影响,但与ECT合用则可改善ECT后的学习记忆;ECT干预与药物干预存在交互作用。ECT可明显增加海马中Glu浓度;异丙酚和人参皂苷Rg-1在ECT前后均可减少Glu浓度;且ECT干预与药物干预存在交互作用。PP-2A和氯化锂在ECT前后对Glu浓度无明显影响。结论 ECT可使海马Glu浓度升高,导致学习记忆障碍;异丙酚与人参皂苷Rg-1、PP-2A和氯化锂均可改善ECT后的学习记忆;前两者与降低海马中Glu浓度有关,后两者与此无关。Objective To explore and compare the effects of propofol,ginsenoside Rg-1,protein phosphatae-2A,and lithium on the learning and memory and the concentration of glutamic acid in hippocampus after the electmconrulsive therapy （ECT） in the model of depressed rats induced after the removal of olfactory bulb.Methods The depressed rats were randomized into ECT intervention （two levels：no disposition and a course of electroconvulsive shock） and drug intervention （five levels：microinjection of saline injection,propofol,ginsenoside Rg-1,protein phosphatae-2A,and lithium,20 g/L）.Learning and memory were evaluated using the Morris water maze test within 24 h after the course of ECT.Glutamate contents in the hippocampus of rats were examined using high-performance liquid chromatography.Results Both propoM alone and ECT alone induced the impairment of learning and memory in depressed rats,but their combination alleviated the such impairment caused by ECT.Ginsenoside Rg-1,protein phosphatae-2A,and lithium had no obvious effect on the leaning and improved the learning and memory when in combination with ECT.There was a synergic effect between ECT intervention and drug intervention.ECT remarkably increased the glutamate content in the hippocampus of depressed rats,which could be reduced by both propoM and ginsenoside Rg-1.Protein phosphatae-2A and lithium did not affect glutamate content in the hippocampus of depressed rats before and after ECT.Conclusions ECT can increase the content of glutamate in hippocampus and thus cause the impairment of learning and memory in depressed rats.PropoM and ginsenoside Rg-1 can ameliorate the impairment by reducing the content of glutamate in hippocampus.Protein phosphatae-2A and lithium may also improve the learning and memory in depressed rats.

关 键 词：异丙酚 人参皂苷Rg-1 蛋白磷酸酯酶-2A 氯化锂 电休克 学习记忆能力 谷氨酸 

分 类 号：R395.1[哲学宗教—心理学]