抗磷脂抗体促进ox-LDL诱导U937细胞泡沫化进程及对VEGF分泌的影响  

Effects of antiphospholipid antibody on the transformation from U937 cell into foam cell and VEGF secretion induced by ox-LDL

作　　者：白玲[1] 刘平[1] 马爱群[1] 杜媛[1] 霍建华[1] 范力宏[1] 强华[1] 

机构地区：[1]西安交通大学医学院第一附属医院心血管内科,环境与疾病相关基因教育部重点实验室,陕西省分子心脏病学重点实验室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2014年第4期429-432,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81270235);陕西省科技攻关项目(No.2012K15-01-01)~~

摘　　要：目的通过观察抗磷脂抗体(APA)对氧化低密度脂蛋白(ox-LDL)诱导U937细胞泡沫化进程及血管内皮生长因子(VEGF)分泌的影响,以探讨APA致动脉粥样硬化(AS)的机制。方法利用细胞培养、酶荧光法、透射电镜、DNA凝胶电泳及ELISA方法,观察在ox-LDL存在条件下APA对人类单核细胞株U937内胆固醇、胆固醇酯的含量、细胞凋亡、VEGF蛋白分泌的影响。结果 ox-LDL(80mg/g细胞)作用48h,U937细胞内胆固醇[(215±10)mg/g细胞vs.(243±7)mg/g细胞]、胆固醇酯[(87±10)mg/g细胞vs.(226±12)mg/g细胞]明显增加,但未达到泡沫化细胞,也未出现凋亡现象;在加入APA后,胞质内胆固醇[(276±10)mg/g细胞]、胆固醇酯[(384±13)mg/g细胞]进一步增加,生化指标及形态学改变均显示达泡沫化程度,且出现凋亡细胞;同时细胞分泌VEGF蛋白的水平由(2 331±178)pg/mL增加到(2 716±145)pg/mL。结论 APA可以促进U937摄取ox-LDL,诱使细胞出现凋亡现象,加速U937细胞的泡沫化进程和提高VEGF的分泌。Objective To investigate the mechanisms of antiphospholipid antibody （APA） inducing atherosclerosis （AS） by observing the effects of APA on the formation of macrophage-derived foam cell line from human U937 cell line induced by oxidized low density lipoprotein （ox-LDL） and the secretion of vascular endothelial growth factor （VEGF）.Methods U937 cells were incubated with ox-LDL or ox-LDL and APA together to induce the transformation of U937 into foam cells.The contents of cholesterol and cholesterol ester,apoptosis,and secretion of VEGF were observed by cell culture method,enzyme-fluorometric method,transmission electron microscopy,DNA gel electrophoresis and enzyme-linked immunosorbent assay.Results After incubation with oxLDL,the contents of cholesterol and cholesterol ester in U937 cells increased from （215± 10）mg/g cell protein and （87±10）mg/g cell protein to （243±7）mg/g cell protein and （226±12）g/g cell protein,respectively.When the U937 cells were incubated with APA and ox-LDL together,the contents of cholesterol and cholesterol ester increased to a higher level of （276± 10）mg/g cell protein and （384± 13）mg/g cell protein; foam cells and apoptotic cells appeared.The secretion of VEGF also increased from （2 331±178）pg/mL to （2 716±145）pg/mL.Conclusion APA can promote the intake of ox-LDL by U937 cells,induce apoptosis,accelerate the transformation of foam cells,and elevate the secretion of VEGF.

关 键 词：抗磷脂抗体 氧化低密度脂蛋白 泡沫细胞 血管内皮生长因子 凋亡 

分 类 号：R543.5[医药卫生—心血管疾病]