坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡  

Transfusion of necrotic cells redresses regulatory T cell and Th17 cell imbalance in septic mice

作　　者：顾冬生[1,2] 熊露[3] 韩艳萍[1] 孙尔维[1] 

机构地区：[1]南方医科大学第三附属医院风湿免疫科,广东广州510630 [2]解放军第421医院泌尿外科,广东广州510318 [3]广州爱尔眼科医院,广东广州510080

出　　处：《南方医科大学学报》2014年第7期956-960,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81273310);国家自然科学基金青年基金(81202359)~~

摘　　要：目的研究预输注坏死细胞对脓毒症小鼠模型Treg/Th17细胞平衡的影响。方法将34只C57BL/6小鼠随机分成4组:正常对照组(PBS组,n=5)、假手术对照组(Sham组,n=5)、脓毒症组(CLP组,n=12)及坏死细胞处理组(Nec组,n=12)。PBS组为正常小鼠,腹腔注射200μl PBS一次,Sham组小鼠手术分离盲肠末端后,不进行结扎穿孔;CLP组小鼠用盲肠结扎穿孔法(CLP)建立脓毒症模型;Nec组小鼠预先腹腔注射2×107个坏死细胞,第5天再行盲肠结扎穿孔。所有小鼠于制备CLP模型后2周处死,流式细胞仪检测外周血、脾脏和胸腺中CD4+Foxp3+Treg细胞以及CD4+IL17A+Th17细胞比例。结果 CLP组脾脏中Th17比例及Treg/Th17比值均显著高于PBS组及sham组(P<0.01);CLP组小鼠胸腺内Treg比例显著低于Sham组(P<0.01);而预输注坏死细胞,可逆转上述CLP手术后小鼠体内Th17比例、Treg比例及Treg/Th17的比例失衡(P<0.05)。结论预输注坏死细胞可以逆转脓毒症小鼠体内Treg/Th17细胞亚群的失衡。Objective To investigate the effect of transfusion of necrotic cells on regulatory T （Treg） and Th17 cell balance in septic mice. Method Thirty-four C57BL/6 mice were randomized into PBS group （n=5）, sham-operated group （n=5）, sepsis group （n=12）, and necrotic cell transfusion group （n=12） and subjected to intraperitoneal PBS injection, sham operation by separating the cecum only, cecal ligation and puncture （CLP）, and injection of 2 × 10^7 necrotic cells 5 days before CLP, respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4^＋Foxp3^＋Treg cells and CD4^＋IL17A^＋Th17 cells in the peripheral blood, spleen and thymus by flow cytometry. Results The percentage of Th17 cells and Treg/Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group （P〈0.01）. The percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group （P〈0.01）. Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice following CLP （P〈0.05）. Conclusion Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice.

关 键 词：脓毒症 调节性T细胞 TH17细胞 坏死细胞 

分 类 号：R459.7[医药卫生—急诊医学]