人胚胎源性人绒毛促性腺激素在胚胎培养液中的水平与早期胚胎发育的关系  

Association of human chorionic gonadotropin level in embryo culture media with early embryo development

作　　者：王海英[1] 张仁礼[1] 韩冬[1] 刘彩霞[1] 蔡佳杰[1] 毕燕玲[2] 闻安民[1] 全松[3] 

机构地区：[1]广东省人民医院//广东省医学科学院妇产科生殖医学科,广东广州510080 [2]广东省人民医院//广东省医学科学院检验科,广东广州510080 [3]南方医科大学南方医院妇产科生殖中心,广东广州510515

出　　处：《南方医科大学学报》2014年第7期1039-1041,1047,共4页Journal of Southern Medical University

基　　金：卫生行业科研专项基金(201002013)

摘　　要：目的探讨人胚胎源性人绒毛促性腺激素(HCG)在第3天胚胎培养液中的表达及其与早期胚胎发育间的关系。方法收集IVF-ET患者体外第3天单个培养胚胎培养液,通过ELISA方法检测培养液中HCG浓度,比较其与胚胎数目、分级、碎片等传统评分间的关系。结果在60个病人的163份第3天胚胎培养液标本中,胎源性HCG的检测率是93.8%(153/163),表达浓度是0.85±0.43 mIU/ml。HCG浓度与胚胎卵裂球细胞数呈正相关。随着细胞数增加,培养液中HCG表达也提高(P<0.05)。随着胚胎形态评分级别的下降,培养液中HCG表达下降(P<0.05)。结论 ELISA方法能检测出第3天胚胎培养液中胎源性HCG的微量表达。胚胎培养液中胎源性HCG水平与传统胚胎形态评估有相关性,提示它有可能作为新的评估胚胎发育的无创性方法。Objective To investigate the association of human chorionic gonadotropin （HCG） level on day 3 of embryo culture with embryo development. Methods Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer （IVF-ET） cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed. Results In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample （93.8%） with a mean level of 0.85±0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased （F=2.273, P=0.03）, and decreased as the morphological grade of the embryo was lowered （F=3.900, P=0.02）. Conclusion ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.

关 键 词：胚胎源性 人绒毛膜促性腺激素 胚胎培养液 胚胎评分 体外受精 

分 类 号：R711.6[医药卫生—妇产科学]