慢性间歇性缺氧对大鼠骨骼肌葡萄糖转运蛋白4表达的影响  

Effects of chronic intermittent hypoxia on glucose transporter 4 expression in rat skeletal muscles

作　　者：谭健[1] 莫海兰[1] 李洁[1] 吴应玲[1] 何晓丽[1] 李兵[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016

出　　处：《南方医科大学学报》2014年第7期1061-1064,共4页Journal of Southern Medical University

基　　金：国家临床重点专科建设项目经费基金资助(卫办医政函[2012]649号);重庆市卫生局重点科研项目(2012-1-059)

摘　　要：目的探讨慢性间歇性缺氧所致炎症因子以及复氧对大鼠糖代谢及骨骼肌葡萄糖转运蛋白4(GLUT-4)表达的影响。方法24只SD雄性大鼠,分为空白组(UC组)、慢性间歇性缺氧组(CIH组)和复氧组(RH组);所有大鼠在模型建立后,分别用氧化酶-过氧化物酶法、放射免疫法、ELISA检测血糖、血清胰岛素及炎症因子的变化;Western blotting检测骨骼肌GLUT-4蛋白的表达。结果大鼠空腹血糖,CIH组高于UC组和RH组(P<0.05),RH组高于UC组(P<0.05);血清胰岛素及胰岛素抵抗指数,CIH组高于UC组和RH组(P<0.05)。各组大鼠血清炎症指标TNF-α、IL-6,CIH组显著高于UC组和RH组(P<0.05),RH组高于UC组(P<0.05)。大鼠骨骼肌GLUT4蛋白,CIH组显著低于UC组和RH组(P<0.05),RH组低于UC组(P<0.05)。结论慢性间歇性缺氧可引起大鼠体内炎症因子增加及胰岛素抵抗;大鼠胰岛素抵抗与炎症因子所致的骨骼肌GLUT-4蛋白量降低有关。Objective To study the effect of chronic intermittent hypoxia-induced inflammatory cytokines and reoxygenation on glucose transporter 4 （GLUT-4） expression in rat skeletal muscles. Methods Twenty-four male Sprague-Dawley rats were randomly assigned to blank control group, chronic intermittent hypoxia （CIH） group, and reoxygenation group. At the end of the experiment, fasting blood glucose （FBG）, fasting blood insulin （FINS） and serum inflammatory cytokine levels were measured with glucose oxidase-peroxidase, insulin radioimmunoassay and ELISA, respectively. Homeostasis model assessment （IRI） was used to evaluate insulin resistance in the rats, and GLUT-4 protein expression in the skeletal muscles was measured with Western blotting. Results Compared with the blank control group, CIH resulted in significantly increased fasting blood glucose, blood insulin levels and insulin resistance index （IRI） （P〈0.05）; fasting blood glucose was significantly elevated in reoxygenation group （P〈0.05）. Inflammatory cytokines levels （IL-6 and TNF-α） were significantly higher in CIH group than in the blank control and reoxygenation groups （P〈0.05）, and were higher in reoxygenation group than in the blank control group. GLUT-4 expression in the skeletal muscles was significantly reduced after CIH （P〈0.05） but increased after subsequent reoxygenation （P〈0.05）. Conclusions CIH can cause increased release of inflammatory cytokines to lower GLUT-4 protein expression in the skeletal muscles, which contributes to insulin resistance in adult rats.

关 键 词：睡眠呼吸紊乱 慢性间歇性缺氧 炎症因子 糖代谢 胰岛素抵抗 葡萄糖转运蛋白4 

分 类 号：R766[医药卫生—耳鼻咽喉科]