Endoderm contributes to endocardial composition during cardiogenesis  

作　　者：Yan Li Xiaoyu Wang Zhenglai Ma Manli Chuai Andrea Münsterberg Kenneth KaHo Lee Xuesong Yang 

机构地区：[1]Key Laboratory for Regenerative Medicine, Ministry of Education, Medical College, Jinan University [2]Division of Cell and Developmental Biology, University of Dundee, Dundee DD1 5EH, UK [3]School of Biological Sciences, University of East Anglia,Norwich NR4 7TJ, UK [4]Stem Cell and Regeneration Thematic Research Programme,School of Biomedical Sciences, Chinese University of Hong Kong

出　　处：《Chinese Science Bulletin》2014年第22期2749-2755,共7页

基　　金：supported by the National Natural Science Foundation of China (30971493, 31071054);the National Basic Research Program of China (2010CB529703)

摘　　要：Heart formation commences from a single heart tube,which fuses from bilateral primordial heart fields.The developing heart tube is composed of outer-layer myocardial cells and inner-layer endocardial cells.Several distinct populations of precardiac cells contribute to cardiac morphogenesis.However,it still remains not very clear about the lineage of endocardium at gastrulation stage.Thereby,this study focused on ascertaining the correlation between the hypoblast in gastrulation and endocardium during cardiogenesis.Firstly,the fusing heart tube morphologically is closed to endoderm-derived pharynx floor,implying the possibility that pharynx floor might be wrapped into the formation of endoderm.Secondly,HNK1 is expressed in hypoblast strongly at gastrula stage and subsequently appeared in endocardium of cardiogenesis.Moreover,fate map data displayed that DiI labeled hypoblast was also present in endocardium later on.One more evidence is chick-quail chimera of hypoblast transplantation,in which quail-hypoblast derivative could be identified inendocardium of cardiogenesis by QCPN antibody.In sum,our current data suggests that endoderm in gastrula contribute at least partly to the formation of endocardium of cardiogenesis.Heart formation commences from a single heart tube, which fuses from bilateral primordial heart fields. The developing heart tube is composed of outerlayer myocardial cells and innerlayer endocardial cells. Several distinct populations of precardiac cells contribute to cardiac morphogenesis. However, it still remains not very clear about the lineage of endocardium at gastrulation stage. Thereby, this study focused on ascertaining the correlation between the hypoblast in gastrulation and endocardium during cardiogenesis. Firstly, the fusing heart tube morphologically is closed to endoderm-derived pharynx floor, implying the possibility that pharynx floor might be wrapped into the formation of endoderm. Secondly, HNK1 is expressed in hypoblast strongly at gastrula stage and subsequently appeared in endocardium of cardiogenesis. Moreover, fate map data displayed that DiI labeled hypoblast was also present in endocardium later on. One more evidence is chickquail chimera of hypoblast transplantation, in which quailhypoblast derivative could be identified in endocardium of cardiogenesis by QCPN antibody. In sum, our current data suggests that endoderrn in gastrula contribute at least partly to the formation of endocardium of cardiogenesis.

关 键 词：心内膜 心脏 胚层 原肠胚期 组成 心肌细胞 数据显示 膜细胞 

分 类 号：Q954.4[生物学—动物学]