大肠杆菌Ⅰ型分泌表达系统研究进展及提高蛋白表达量的策略  

Research Advances in Secretary Production of Recombinant Protein Using Escherichia coli Type I Secretion System and Strategies for Enhancement of Secretion of Type I Pathway

作　　者：王靖瑶[1] 王天女[1] 卢磊[1] 张帅[1] 赵敏[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《中国生物工程杂志》2014年第6期98-104,共7页China Biotechnology

基　　金：国家自然科学基金资助项目(31170553;31200394)

摘　　要：大肠杆菌是表达重组蛋白最常用的宿主之一。利用大肠杆菌分泌途径胞外表达重组蛋白具有可促进蛋白正确折叠,有效减少包涵体形成,简化纯化工序等诸多优势,近年来备受关注。其中,大肠杆菌Ⅰ型分泌途径具有分泌表达速度快,蛋白活性高,对宿主代谢无影响等特点,是目前应用最广泛的分泌途径之一。综述了大肠杆菌Ⅰ型分泌系统的元件组成和分泌机理及提高Ⅰ型分泌系统蛋白表达量的有效策略,为重组蛋白生产应用提供了理论依据。Escherichia coli is one of the most commonly used hosts for recombinant protein production. Extracellular protein expression in E. coli, which is favored by its improved correct folding of target protein, reduced formation of inclusion bodies and simplified purification process, is attracting increasing attention. Among all the secretion systems of E. coli, the type I secretion system has become one of the most popular secretion pathways due to its fast secretion speed, high expression ability and harmlessness to cell physiology. The components and mechanism of type I pathway of E. coli and efficient strategies for enhancing secretion efficiency of recombinant protein are summarized, which provides the theoretical basis for production of recombinant protein.

关 键 词：大肠杆菌 胞外表达 重组蛋白 Ⅰ型分泌途径 高效分泌策略 

分 类 号：Q786[生物学—分子生物学]