双链RNA对鼻上皮细胞表达上皮中性粒细胞活化肽78的诱导作用  

Double-stranded RNA promotes the expression of epithelial neutrophil activating peptide 78 in human airway epithelial cells

作　　者：张涵[1] 陈曦[1] 韦屹[1] 李华斌[1] 

机构地区：[1]中山大学附属第一医院耳鼻咽喉科医院,广东广州510080

出　　处：《中国耳鼻咽喉头颈外科》2014年第4期205-208,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金项目(81271054)

摘　　要：目的观察双链RNA对离体培养的气道上皮细胞表达上皮中性粒细胞活化肽（epithelial neutrophil activating peptide,ENA）78的诱导作用。方法离体培养的鼻息肉上皮细胞（polyp epithelial cells,PECs）和人支气管上皮细胞（human bronchial epithelial cells,HBECs）经过双链RNA模拟物聚肌胞苷酸（Polyinosinic：polycytidylic acid,Poly I：C）刺激0~24h后,通过定量聚合酶链反应（quantitative-polymerase chain reaction,Q-PCR）和酶联免疫吸附试验（enzyme linked immunosorbent assay,ELISA）检测ENA78 mRNA和蛋白水平的表达变化;通过Transwell技术检测Poly I：C诱导的ENA78对中性粒细胞的趋化作用。结果 Poly I：C刺激后,PECs和HBECs 2类气道上皮细胞ENA78 mRNA和蛋白的表达显著增高（t=21.7、19.8,P均=0.001）。Poly I：C诱导的HBECs上清对中性粒细胞存在显著的趋化作用（t=20.8,P=0.001）,后者能被加入ENA78抗体显著抑制（t=12.9,P=0.001）。结论双链RNA能诱导气道上皮细胞表达ENA78进而诱导中性粒细胞趋化,抑制ENA78可作为干预由病毒感染导致的鼻息肉组织中性粒细胞聚集的一个关键靶点。OBJECTIVE To evaluate the effect of double-stranded RNA on the expression of epithelial neutrophil activating peptide（ENA） 78 in cultured human airway epithelial cells.METHODS Cultured polyp epithelial cells（PECs） and human bronchial epithelial cell（HBECs） were stimulated with Polyinosinic：polycytidylic acid（Poly I：C） for0-24h.After that,ENA78 mRNA and protein level were examined by Q-PCR and ELISA,and chemotaxis of neutrophil in the presence of ENA78 and specific antibody were evaluated in Transwell culture system.RESULTS We found Poly I：C significantly upregulated ENA78 expression in cultured PECs and HBECs（P0.01）.After Poly I：C incubation,supernatants of HBECs were found to significantly enhance neutrophil chemotaxis index,which was significantly inhibited by adding ENA78 antibody（P0.01）.CONCLUSION Poly I：C is able to induce ENA78 in human airway epithelial cells which subsequently promoted neutrophil chemotaxis,and blocking ENA78 may represent a promising therapeutic for neutrophilic airway diseases.

关 键 词：鼻黏膜 上皮细胞 中性白细胞 病毒 上皮中性粒细胞活化肽78 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]