钙敏感受体及紧密连接蛋白-14在草酸钙结石大鼠肾组织中的表达及意义  

Expressions of CaSR and Claudin-14 in Renal Calcium Oxalate Stone Model of Rats

作　　者：孙稳[1] 王勤章[2] 丁国富[2] 

机构地区：[1]石河子大学医学院,832000 [2]石河子大学医学院第一附属医院泌尿外科

出　　处：《天津医药》2014年第7期657-660,共4页Tianjin Medical Journal

基　　金：国家科技支撑计划项目(2013BAI05B05)

摘　　要：目的初步探讨钙敏感受体(CaSR)和紧密连接蛋白(Claudin)-14在肾草酸钙结石大鼠肾组织中的表达及意义。方法 30只雄性SD大鼠随机分为对照组和结石组各15只,采用1%乙二醇和2%氯化铵每日2 mL灌胃诱导制作大鼠肾草酸钙结石模型。免疫组织化学检测CaSR蛋白表达;RT-PCR检测Claudin-14 mRNA表达;West-ern-Blotting分别检测CaSR和Claudin-14蛋白表达;全自动生化分析仪检测2组大鼠肾功能、血及尿生化指标。结果光镜下结石组有大量结石结晶形成,血清肌肝(Cr)、尿素氮(BUN)、24 h尿钙及尿量较对照组升高(P<0.01),2组间血钙、尿pH差异无统计学意义。结石组Claudin-14 mRNA及CaSR蛋白表达较对照组升高(P<0.01),Claudin-14蛋白在结石组大鼠肾组织中呈特异性高表达,对照组未检测到Claudin-14蛋白表达;结石组大鼠肾组织中CaSR和Claudin-14蛋白表达呈正相关,同时,CaSR、Claudin-14蛋白表达与24 h尿钙排泄量也呈正相关。结论 CaSR和Claudin-14表达增高可通过增加尿钙排泄量,进而参与结石的形成。Objective To investigate the expressions of calcium sensitive receptor （CaSR） and tight junction protein （Claudin）-14 in renal calcium oxalate stone rat model. Methods Thirty male SD rats were randomly divided into control group （n=15） and model group （n=15）. The rat model of renal calcium oxalate stone was established by gavaging 1%glycol （2 mL/d） and 2% ammonium chloride. The expression of CaSR protein was detected using immunohistochemical assay. RT-PCR was used to detect the Claudin-14 mRNA expression. The expression levels of CaSR and Claudin-14 protein were de-tected by Western blot assay respectively. The full automatic biochemical analyzer was used to detect rat renal function and changes of blood and urine biochemical indices. Results A large stone crystallization was observed under light microscope in model group. The serum levels of Cr, BUN, 24-h urine calcium and urine volume were significantly higher in model group than those of control group （P〈0.01）. There were no significant differences in serum calcium level and urinary pH value be-tween two groups. The expression levels of Claudin-14 mRNA and CaSR protein were significantly higher in model group than those of control group （P〈0.01）. The Claudin-14 protein expression was specifically higher in renal tissues of model group. There was no Claudin-14 protein expression in control group. There was a positive correlation between CaSR and Claudin-14 protein expression in renal tissues of model group. Also, CaSR and Claudin-14 protein expressions were posi-tively correlated with the 24-h urine volume. Conclusion The increased expressions of CaSR and Claudin-14 involved in the formation of kidney stone by increasing the urinary calcium excretion.

关 键 词：肾结石 草酸钙 受体 钙敏感 大鼠 SPRAGUE-DAWLEY 紧密连接蛋白-14 

分 类 号：R692.4[医药卫生—泌尿科学]