梯形面积法研究蛇床子素在大鼠体内组织分布  

Characterization on Tissue Distribution of Osthol In Vivo in Rats by Trapezoidal Area Method

作　　者：郑立卿[1] 刘建华[2] 张丹参[1] 武海霞[1] 薛贵平[1] 

机构地区：[1]河北北方学院药理教研室 [2]河北北方学院附属第一医院呼吸内科

出　　处：《神经药理学报》2012年第4期13-18,共6页Acta Neuropharmacologica

基　　金：河北省卫生厅医学科学研究重点课题计划项目(No.20120155;No.20110173);河北省中医药管理局项目(No.2009065)

摘　　要：目的:研究蛇床子素(osthol,Ost)在大鼠体内的组织分布特征,为临床合理用药提供合理依据。方法:大鼠腹腔注射(intraperitoneal injection,ip)30,120 mg·kg-1蛇床子素后,采用RP-HPLC法测定大鼠组织中不同时间点的药物浓度,分析蛇床子素在大鼠体内的组织分布特点。结果:大鼠腹腔注射蛇床子素后,组织分布较快,给药5 min 8种组织中均可检测到蛇床子素,10 min内心、肝、肺、肾均可达到峰值。当给药浓度增加4倍时,蛇床子素在各组织中的达峰时间没有显著性变化,只是相应时间点的药物浓度均略有升高,药物消除的时间也相应延长。结论:蛇床子素在大鼠组织中分布快、广泛,消除也快,且可能穿越血脑屏障和血睾屏障。Objective：To study the tissue distribution of osthol on rats and to provide bases for clinical applications. Methods：After osthol was administered to rats（30,120 mg·kg-1 ip）,the concentration of osthol in rat tissues after different time was determined by RP-HPLC. Results：Osthol distributed rapidly in the tissues after ip：eight tissues can detected osthol after 5 min. Heart,liver, lung and kidney all reached Cmax in 10 min. When the dose is increased 4 folds,Tpeaks of osthol in tissues had no significant changes,except the concentration and elimination time increased slightly corresponding to the same time-point. Conclusion：Osthol was distributed rapidly and extensively in the rat’s body and may pass through blood-brain-barrier and blood-testis-barrier.

关 键 词：蛇床子素 大鼠 组织分布 

分 类 号：R965[医药卫生—药理学]