6-羟多巴胺诱导帕金森病大鼠模型行为学评价方法的探讨  

Assessment of 6-hydroxydopamine-Lesion Induced Behavioral Alteration as a Rat Model of Parkinson's Disease

作　　者：杨谦谦 孙芳龄 艾厚喜[2] 张丽[2] 王文[2] 

机构地区：[1]河北北方学院药学系 [2]首都医科大学宣武医院药物研究室

出　　处：《神经药理学报》2013年第1期17-22,共6页Acta Neuropharmacologica

基　　金："重大新药创制"科技重大专项项目(No.2012ZX09102-201-016);国家自然科学基金项目(No.81173575);北京市自然科学基金项目(No.7062031);北京市教委科技创新平台项目(No.111219)

摘　　要：目的:复制帕金森病(Parkinson's disease,PD)大鼠模型并根据行为学检查结果对此模型进行较为全面的评价,以期建立治疗此疾病的新型实验平台。方法:运用6-羟多巴胺(6-hydroxydopamine)单点定向注射黑质-纹状体的方法,损毁SD大鼠左侧中脑多巴胺能神经元,动物术后3周腹腔注射(intrapertioneal injection,ip)阿扑吗啡(apomorphine,APO)观察是否诱导动物向健侧旋转行为,复制PD模型。分别应用转棒实验和旷场实验分析测定动物的运动功能,应用肌电测试实验测定大鼠的震颤频率和幅度,并据此对动物模型进行评价和分类。结果:造模后,部分大鼠转棒实验在棒时间显著缩短,旷场实验横向跨格次数减少,出现运动功能障碍;肌电检测结果显示部分大鼠肌肉震颤频率和幅度均增加。结论:行为学测试为PD动物模型的评价以及进行不同类型的分组提供了实验依据。Objective：To systematically evaluate 6-hydroxydopamine（6-OHDA）-induced behavioral alteration as a rat model of Parkinson’s disease. Methods：6-OHDA was microinjected into the left side of the substantia nigra striatum to damage the dopaminergic neurons in the SD rats. Three weeks later,intraperitoneal injection of apomorphine（APO）to observe the rotational behavior. The motoric function of animals was analyzed with rotarod test and open field test, and the rat’s muslce vibration frequency and amplitude were determined using the myoelectricity test. The severity of the behavioral alterations of the individual animals was also categorized. Results：The time of rats that remained on the rotarod was significantly reduced in model group as compared to sham group. In the open field test,the horizontal travel distance was decreased in the model group. The myoelectricity test result showed that the muscle vibration frequency and amplitude was increased in animals receiving 6-OHDA microinjection. Conclusion：These results provided behavioral evidence in future studies to evaluate and categorize Parkinson-like＆nbsp;behaviors in rats.

关 键 词：帕金森病 动物模型 行为学 评价方法 

分 类 号：R965[医药卫生—药理学]