类风湿关节炎患者外周血IL-25的表达及临床意义  

Expression level and clinical significance of interleukin-25 in peripheral blood of rheumatoid arthritis patients

作　　者：唐宏勇[1] 李忆农[2] 洪春梅[1] 

机构地区：[1]福建医科大学福总临床医学院(南京军区福州总医院)风湿免疫科,福州350000 [2]南京军区福州总医院风湿免疫科,福州350000

出　　处：《新医学》2014年第7期472-476,共5页Journal of New Medicine

摘　　要：目的检测类风湿关节炎(RA)患者外周血IL-25和IL-17表达水平,同时分析其与临床指标及疾病活动度评分(DAS28)的关系,初步探讨IL-25在RA中的临床意义。方法收集年龄大于16岁的80例RA患者及30名健康志愿者外周血标本并分组,DAS28>3.2为RA活动组,DAS28≤3.2定义为RA低缓组,并进行双手关节X线Sharp总分评分及健康评估问卷(HAQ)调查;采用实时荧光定量(QRT)-PCR检测外周血单个核细胞(PBMC)中的IL-25和IL-17的mRNA表达水平,ELISA检测血清IL-25、IL-17的蛋白浓度,并分析其与临床指标的关系;采用单因素方差分析、Spearman或Pearson相关分析和多元回归分析进行统计学处理。结果 RA活动组患者血清IL-25和IL-17蛋白浓度高于RA低缓组和健康组(P<0.01),RA低缓组与健康组比较差异无统计学意义(P均>0.05)。RA活动组患者PBMC中IL-25和IL-17的mRNA表达水平均高于RA低缓组和健康组(P均<0.01),低缓组与健康组比较差异无统计学意义(P均>0.05)。RA活动组患者血清IL-25的蛋白浓度与CRP、ESR、RF、抗CCP抗体、DAS28、Sharp总分、HAQ评分均呈正相关(P<0.01),外周血IL-25 mRNA的表达水平亦与上述指标呈正相关(P<0.05)。RA患者外周血IL-25的mRNA表达水平及蛋白浓度与IL-17相应水平有关(b=0.748,P<0.01;b=0.843,P<0.01)。结论 IL-25有可能参与RA的免疫炎症反应,亦有可能作为RA活动性参考指标之一。Objective To measure the expression levels of interleukin （IL） -25 and IL-17 in peripheral blood of patients with rheumatoid arthritis （RA） and analyze the relationship between 1L-25, and clinical index and the disease activity index 28 （ DAS 28 ） scores in RA patients. The aim of this study is to identify the clinical significance of IL-25 in RA patients. Methods Peripheral blood samples were collected from 80 RA patients and 30 healthy volunteers. All subjects were above 16 years old. Fifty RA patients with DAS 28 score 〉 3.2 were assigned into the RA active group and 30 with DAS 28 score ≤3.2 were allocated into the RA inactive group. Hands joint X-ray total Sharp score and Health Assessment Questionnaire （HAQ） were recorded. The mRNA levels of IL-25/IL-25R and IL-17 in the peripheral blood mononuclear cells （PBMC） were determined by quantitative real-time PCR （QRT-PCR）. The serum IL-25 and IL-17 proteins were detected by enzyme-linked immunosorbent assay （ELISA）. One-way ANOVA, Spearman＇s or Pearson＇s colTelation and multiple linear regression were adopted to perform statistical analysis. Results The serum IL-25 and IL-17 proteins in the RA active group was significantly higher than those in the RA inactive and control groups （P 〈 0. 01 ）, whereas no statistical significance was noted between the RA inactive and control groups （ P 〉 0.05 ）. The mRNA levels of IL-25 and IL-17 in the RA active group were higher compared with those in the RA inac- tive and control groups （ P 〈 0. 01 ） , whereas no significant difference was found between the RA inactive and control groups （P 〉 0. 05）. The potein level of serum IL-25 was positively correlated with the levels of CRP,ESR, RF, anti-CCP antibody, DAS 28, total Sharp score, HAQ （P 〈 0. 01 ）, and the same outcome was found in the mRNA levels of IL-25 in PBMC （P 〈 0. 05）. Both mRNA and protein levels of IL-25 were posi- tively correlated with corresponding IL-17 levels （b = 0. 748, P 〈 0. 01 ; b

关 键 词：关节炎 类风湿 白细胞介素-25 相关性 

分 类 号：R593.22[医药卫生—内科学]