风湿性瓣膜病心房颤动患者心房肌HCN2基因mRNA及蛋白的表达  

The mRNA and protein expression levels of atrial hyperpolarization-activated cyclic nucleotide-gated channel 2 in atrial fibrillation patients with rheumatic heart disease

作　　者：孙宇[1] 刘文洲[1] 冯旭[1] 钱俊[1] 赵春鹤[1] 周华富[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,广西南宁530021

出　　处：《中国心脏起搏与心电生理杂志》2014年第3期246-248,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：广西自然科学基金资助项目(编号:2012GXNSFAA053094);广西科技攻关计划资助项目(编号:0816004-24)

摘　　要：目的研究风湿性心脏瓣膜病(简称风心病)心房颤动(简称房颤)患者心房肌超极化激活环化核苷酸门控通道-2(HCN2)基因mRNA及蛋白表达的变化。方法选取风心病二尖瓣狭窄患者38例,根据是否合并房颤将其分为两组,房颤组(n=21),窦性心律组(n=17),术中取右心耳组织,应用逆转录聚合酶链反应及蛋白质免疫印迹法分别检测心房肌HCN2基因mRNA及蛋白的表达水平,并分析其与左右房内径的关系。结果与窦性心律组比较,房颤组心房肌HCN2 mRNA表达增加[(0.613±0.179)vs(0.439±0.158),P<0.01],其蛋白表达亦增加[(0.431±0.195)vs(0.225±0.121),P<0.01],但其表达与左右房内径无相关关系。结论风心病房颤患者心房肌HCN2基因表达上调。Objective To study the mRNA and protein expression levels of hyperpolarization-aetivated cyclic nueleotide-gated channel 2 （HCN2） channel in muscles of right atrium in rheumatic heart disease patients with atrial fibrillation （AF）. Methods Thirty-eight rheumatic heart disease patients with mitral valve stenosis were divide into AF group （n = 21 ） and sinus rhythm （SR） group （n = 17） according to basic rhythm. Right atrial appendages were obtained underwent cardiac valve replacement surgery in two groups. The mRNA expression of HCN2 was detected with reverse transcription polymerase chain reacton, and the protein expression was measured with Western bolt. The correlation with the left and right atrial dimension was also analyzed. Results Comparing to SR group, the mRNA and protein expression levels of HCN2 channel were both significantly higher in AF group [ （0. 613±0. 179） vs （0.439±0. 158） and （0. 431±0. 195） vs （0. 225±_0. 121 ）, P〈0.01 ], but were unrelated to the left and right atrial dimension. Conclusion The overexpression of HCN2 gene in AF patients with rheumatic heart disease may participate in the progress of AF.

关 键 词：心血管病学 HCN2通道 心房颤动 风湿性心脏病 

分 类 号：R541.75[医药卫生—心血管疾病] R542.5[医药卫生—内科学]