薄层荧光扫描法测定三颗针中小檗碱的含量  

Determination of Berberine in Berberis by TLC-fluorescence Scanning Method

作　　者：郑彦慧[1] 李国名[1] 陈鹏 

机构地区：[1]石家庄财经职业学院化工制药系,河北石家庄050061 [2]石家庄生产力促进中心,河北石家庄050091

出　　处：《河北师范大学学报（自然科学版）》2014年第4期392-394,共3页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(20675025)

摘　　要：采用薄层荧光扫描法测定了三颗针中的主要成分小檗碱的含量.小檗碱的荧光峰面积与浓度呈良好的线性关系,线性范围为0.010~0.060μg,Y=83.1＋13 721.6 x,r=0.999,平均回收率为97.8%,RSD为2.07%.本方法分离度好、简单、重现性好.The Berberine in Berberis was determinaed by using layer fluorescence scanning method.The berberine was in good linearity from 0.010 to 0.060μg,and the regression equation was Y=83.1＋13 721.6 x（r=0.999）.The mean recovery was 97.8% and RSD was 2.07%,respectively.The method was quick and simple with good repetition.

关 键 词：三颗针 小檗碱 薄层荧光扫描法 

分 类 号：O657.7[理学—分析化学]