广西猪源沙门菌血清学分型及生物被膜形成能力的研究  

Biofilm formation and serotypes of swine Salmonella strains isolated from Guangxi Province

作　　者：蹇慧[1] 黎木兰[1] 艾佛德 刘芳[1] 方小文[1] 杨洋[1] 黄伟坚[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530004

出　　处：《畜牧与兽医》2014年第7期1-5,共5页Animal Husbandry & Veterinary Medicine

基　　金：广西科技攻关项目(1123007-3);广西大学实验教学改革项目(20121705);广西大学动物科技学院院科研项目(DK20110612);广西科学基金项目(桂科自0728015)

摘　　要：为了研究近年广西地区流行猪源沙门菌的血清型和生物被膜形成能力,对从广西区内不同地区规模猪场分离鉴定的35株沙门菌,用玻片凝集法进行血清学分型;应用结晶紫染色微量板定量法检测菌株的生物被膜形成能力。结果显示:试验菌株分属14种血清型,所有菌株均具有生物被膜形成能力:20株弱成膜能力,4株中等成膜能力,11株强成膜能力。其中,鼠伤寒沙门菌的成膜能力较强。In order to investigate the serotypes and biofilm formations of swine Salmonella strains isolated from Guangxi province in recent years, a total of 35 strains were included in the test. The serotypes of isolated strains were detected by slide agglutination, and the biofilm formation ability of the strains was carried out by crystal violet assay on polystyrene micro-well plates. Results showed that the tested strains belong to 14 serotypes. The strains were classified as weak （20 strains）, moderate （4 strains）, and strong （11 strains） biofilm producers. Salmonella enterica serotype Typhimurium produced the most substantial biofilms.

关 键 词：猪 沙门菌 血清型 生物被膜 

分 类 号：S852.6[农业科学—基础兽医学]