疏肝理脾化瘀祛湿方对非乙醇性脂肪肝大鼠的影响  

Effect of Shuganlipihuayuqushi decoction on nonalcoholic fatty liver disease in rats

作　　者：刘晖杰[1] 许丹[2] 郑超[2] 曾浩[2] 吴洪斌[2] 许华[1] 

机构地区：[1]湖北文理学院襄阳市中心医院肿瘤科,湖北襄阳441021 [2]武汉市中医医院消化内科,湖北武汉430014

出　　处：《新乡医学院学报》2014年第7期521-523,527,共4页Journal of Xinxiang Medical University

摘　　要：目的观察疏肝理脾化瘀祛湿方对非乙醇性脂肪肝(NAFLD)大鼠的作用,并探讨其作用机制。方法高脂饲料制备Sprague-Dawley大鼠NAFLD模型,60只大鼠随机分为空白对照组、模型组、小剂量中药治疗组、中剂量中药治疗组和大剂量中药治疗组,分别给药后观察其对大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、胆固醇(TC)、三酰甘油(TG)及肝组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)水平的影响。结果模型组大鼠血清ALT、AST、TC、TG水平均较空白对照组显著升高(P<0.05)。中药中、大剂量治疗组ALT、TC和TG水平显著低于模型组(P<0.05),大剂量组AST水平显著低于模型组(P<0.05)。各中药组的ALT、AST、TC和TG水平随着剂量增加逐渐降低,大剂量组显著低于中、小剂量组(P<0.05)。模型组的SOD及GSH-PX水平较空白对照组显著下降(P<0.05),而MDA水平较空白对照组显著升高(P<0.05);随着中药剂量的增加,与模型组比较,各中药组MDA水平均显著下降(P<0.05),SOD及GSH-PX水平均显著升高(P<0.05),且大剂量中药治疗组MDA、SOD、GSH-Px水平与空白对照组比较差异均无统计学意义(P>0.05)。结论疏肝理脾化瘀祛湿方对NAFLD大鼠具有降脂及保肝的作用,其机制可能与抗氧化、抑制脂质过氧化及有效清除过氧化自由基相关。Objective To observe the effect of Shuganlipihuayuqushi decoction on nonalcoholic fatty liver disease（NAFLD） in rats,and to explore its mechanism of action.Methods High fat diet was used to make NAFLD model of Sprague-Dawley rats,and 60 rats were randomly divided into blank group,model group,small dose of Chinese medicine treatment group,middle dose of Chinese medicine treatment group and large dose of Chinese medicine treatment group.After the decoction was delivered,the alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,total-cholesterol（TC）,triglyceride（TG）,superoxide dismutase（SOD）,malondialdehyde（MDA） and glutathione peroxidase（GSH-PX） of rats were observed respectively.Results Serum ALT,AST,TC and TG levels of rats in model group increased significantly compared with those in blank group（P〈 0.05）.ALT,TC and TG levels in middle dose and large dose Chinese medicine treatment groups were significantly lower than those in model group（P〈 0.05）.AST level in large dose group was significantly lower than that in model group（P〈 0.05）.The ALT,AST,TC and TG levels in Chinese medicine treatment group gradually reduced with the medicine dose increasing,which in high dose group were significantly lower than that in the middle and small dose group（P〈 0.05）.In model group,SOD and GSH-PX levels decreased significantly while the MDA level increased significantly compared with those in blank group（P〈 0.05）.With the increase of dosage,the MDA levels in Chinese medicine groups were significantly lower while the SOD and GSH-PX levels increased significantly compared with those in model group（P〈 0.05）.MDA,SOD and GSH-PX levels in large dose group had no statistically significant difference compared with those in the blank group（P〉0.05）.Conclusion Shuganlipihuayuqushi decoction can reduce lipid and protect liver on NAFLD rats,the mechanism may be related to antioxidation,depression of lipid peroxidation,effective removal of overoxidation free radi

关 键 词：非乙醇性脂肪性肝 大鼠 疏肝理脾化瘀祛湿方 抗氧化 

分 类 号：R965[医药卫生—药理学]