三种对证不同高血压证型的中药复方对SHR影响的蛋白组学比较分析  

Analysis of Renal Protein Expressions of Spontaneously Hypertensive Rat Intervention by Three Different Chinese Medicine Compounds for Hypertension

作　　者：栗源[1] 可燕[1] 蒋嘉烨[1] 李晓军[1] 江永波[1] 

机构地区：[1]上海中医药大学教学实验中心,上海201203

出　　处：《中药材》2014年第5期833-840,共8页Journal of Chinese Medicinal Materials

基　　金：上海市卫生局中医药科研基金(2012J008A);上海高校青年教师培养资助计划(shzy017);上海高校青年教师培养资助计划(ZZszy12039)

摘　　要：目的:考察治疗不同证型高血压的半夏白术天麻汤、地黄饮子和天麻钩藤饮对自发性高血压大鼠肾脏蛋白谱表达差异,并通过主成分分析综合比较,阐述三种复方的作用特点与调控通路,进而分析高血压分型治疗的理论基础。方法:6周龄的自发性高血压大鼠(Spontaneous hypertensive rat,SHR)与正常对照大鼠(Wistar-Kyoto rat,WKY),对给药组的SHR分别灌胃天麻钩藤饮、半夏白术天麻汤和地黄饮子3种复方,监测鼠尾血压变化至24周龄,统一处死提取肾脏组织蛋白并进行双向电泳实验,经PDQuest软件分析得差异蛋白点,结合肾脏蛋白表达谱以主成分分析法(Principal component analysis,PCA)考察三种复方对SHR的作用特点。结果:SHR从6周龄至10周龄血压处于持续升高状态,此时天麻钩藤饮具有良好的降压效果;当血压稳定保持较高水平时,地黄饮子与半夏白术天麻汤的降压效果优于天麻钩藤饮;24周龄时肾脏蛋白谱分析共找出SHR与WKY表达显著不同的84个蛋白点,结合主成分分析,累计主成分提取95.951%,发现天麻钩藤饮和半夏白术天麻汤对于SHR肾脏蛋白点的改变有显著变化趋势,且向WKY趋近,地黄饮子的作用趋势较为分散。结论:在SHR血压升高初期,天麻钩藤饮具有显著的降压效果;当血压稳定保持较高水平,地黄饮子与半夏白术天麻汤的降压效果优于天麻钩藤饮。半夏白术天麻汤和天麻钩藤饮对SHR肾脏蛋白表达的改善有向WKY回归的趋势,地黄饮子作用较为分散。Objective：To observe the differential protein expression profile of three different Chinese medicine compounds on spon- taneous hypertensive rat, which were Banxiabaizhutianma Decoction, Dihuangyinzi Decoction and Tianmagouteng Decoction, and to elab- orate their function characters and regulation pathway using the principal component analysis and analyze the basic theory of different treatment of hypertension. Methods：Give three compounds to 6-week-old SHR（ Spontaneous hypertensive rat）as experimental group and captopril to 6-week-old WKY（ Wistar-Kyoto rat）as control group by gavage. Monitor the blood pressure of rat tail to 24-week-old and then all rats were decapitated. Extracte the rental protein to finish two-dimensional electrophoresis experiments and get the differential protein spots using PDQuest software. Study the three different compounds＇ effect of trend on SHR blood pressure using PCAC principal component analysis. Results：The blood pressure of SHR remained rising from 6-week-old to 10-week-old, when Tianmagouteng Decoc- tion had a significant anti-hypertension effect. But as the blood pressure of SHR remained high, Banxiabaizhutianma Decoction and Di- huangyinzi Decoction performed better in lowering blood pressure. At last, renal protein expression profile analysis showed that there were 84 significantly different proteins between SHR and WKY of 24-week-old and the PCA result showed that the principal component extraction was 95. 951% ,it was sure that Tianmagouteng Decoction and Banxiabaizhutianma Decoction had significantly changed rental protein spots on SHR while Dihuangyinzi Decoction had not. Conclusion ： In the early stages of rising blood pressure, Tianmagouteng De- coction has significant anti-hypertensive effect. But when the blood pressure remain high, Banxiabaizhutianma Decoction and Dihuangy- inzi Decoction are superior to Tanmagouteng Decoction in anti-hypertension. Banxiahaizhutianma Decoction and Tianmagouteng Decoc- tion can significantly improve rental protein s

关 键 词：自发性高血压大鼠 蛋白组学 天麻钩藤饮 半夏白术天麻汤 地黄饮子 

分 类 号：R285.5[医药卫生—中药学]