超声强化微乳提取分离姜黄中总姜黄素的研究  

Study on Extracting and Separating Curcuminoids from Curcuma longa Rhizome Using Ultrasound Strengthen by Microemulsion

作　　者：岳春华 郑礼涛[1] 郭启明[1] 李坤平[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《中药材》2014年第5期880-883,共4页Journal of Chinese Medicinal Materials

基　　金：广东高校优秀青年创新人才培养计划项目(LYM10092);广东药学院大学生创新实验项目(1057313020)

摘　　要：目的:建立利用微乳提取串联浊点浓缩分离姜黄中总姜黄素的新方法。方法:采用分光光度法测定不同油相、乳化剂和助乳化剂对姜黄素的溶解度,利用伪三相图构建微乳处方;进行均匀试验设计优化提取工艺;采用浊点浓缩分离微乳提取液中的姜黄素类成分。结果:油相选用油酸乙酯,乳化剂选用OP乳化剂,助乳化剂选用聚乙二醇400;乳化剂与助乳化剂的质量比为5∶1,微乳液处方为水∶油酸乙酯∶混合乳化剂=0.45∶0.1∶0.45。最优提取工艺为:时间12.5 min,温度52℃,功率360 W,频率400 kHz,液固比40∶1;总姜黄素的得率为92.17%,油层总姜黄素的回收率为86.85%。结论:该研究所确定的微乳处方对总姜黄素有较好的溶解度,提取效果理想,浊点浓缩回收率高。该方法操作简单,适用于姜黄中总姜黄素的提取分离。Objective ： To establish a new method for the extraction and separation of curcuminoids from Curcuma longa rhizome by cloud-point preconcentration using microemulsions as solvent. Methods ： The spectrophotometry was used to detect the solubility of cureu- min in different oil phase, emulsifier and auxiliary emulsifier, and the microemulsion prescription was used for false three-phase figure optimization. The extraction process was optimized by uniform experiment design. The curcuminoids were separated from microemulsion extract by cloud-point preconeentration. Results：Oil phase was oleie acid ethyl ester;Emulsifier was OP emulsifier;Auxiliary emulsifier was polyethylene glycol（peg） 400;The quantity of emulsifier to auxiliary emulsifier was the ratio of 5：1 ;Microemulsion prescription was water-oleic acid ethyl ester-mlxed emulsifier（0.45： 0. 1：0. 45 ）. The optimum extraction process was：time for 12. 5 min, temperature of 52 ℃ ,power of 360 W ,frequency of 400 kHz, and the liquid-solid ratio of 40： 1. The extraction rate of curcuminoids was 92.17% and 86. 85 % in mieroemulsion and oil phase,respectively. Conclusion：Curcuminoids is soluble in this microemulsion prescription with good extraction rate. This method is simple and suitable for curcuminoids extraction from Curcuma longa rhizome.

关 键 词：姜黄 总姜黄素 微乳提取 超声 

分 类 号：R286[医药卫生—中药学]