HPLC法测定活力源口服液中人参茎叶总皂苷的含量  

Determination of Ginsenoside of Stems and Leaves in Huoliyuan Koufuye by HPLC

作　　者：逄焕欢 费砚群 徐凤丽 杨志宏 

机构地区：[1]吉林省食品药品检验所,长春130033 [2]长春卫尔赛生物药业有限公司,长春130033 [3]吉林敖东药业集团延吉股份有限公司,延吉133000 [4]长春经开药业有限公司,长春130033

出　　处：《中国药品标准》2014年第3期202-205,共4页Drug Standards of China

摘　　要：目的：采用HPLC法测定活力源口服液中人参茎叶总皂苷的含量。方法：色谱柱为C18（4.6 mm ×250 mm，5μm）；流动相为乙腈-0.05％磷酸（20∶80）；检测波长为203 nm。结果：人参皂苷Rg1在0.1813～1.816μg范围内与峰面积线性关系良好（ r＝0.9999），平均回收率为96.2％， RSD为1.9％；人参皂苷Re在0.496～4.96μg范围内与峰面积线性关系良好（ r＝0.9999），平均回收率为95.6％，RSD为1.5％。结论：该方法重复性好、操作简便，可用于活力源口服液中人参茎叶总皂苷的质量控制。Objective：To establish an HPLC method for determination of ginsenoside of stems and leaves in Huoliyuan Koufuye.Methods：The analysis was performed on a C18 （4.6 mm ×250 mm,5 μm） column, the mobile phase was acetonitrile-0.05% phosphonic acid solution（20∶80） and the detection wavelength was at 203 nm.Results：The calibration curve of ginsenoside Rg1 was linear in the range of 0.1813-1.816 μg（ r=0.9999 ） , the average recovery was 96.2%and RSD was 1.9%.The linear range of ginsenoside Re was 0.496-4.96 μg （ r=0.9999 ） , the average recovery was 95.6%and RSD was 1.5%.Conclusion：The method is reproducible ,simple and can be used to control the quality of ginsenoside of stems and leaves in Huoliyuan Koufuye .

关 键 词：活力源口服液 人参茎叶总皂苷 高效液相色谱法 含量测定 

分 类 号：R921.2[医药卫生—药学]