四种栲属植物微卫星标记在钩栲中的通用性检测  

The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana( Fagaceae)

作　　者：叶林江 王静[1] 孙鹏[1] 董书鹏 张志勇[1] 

机构地区：[1]江西农业大学亚热带生物多样性实验室,江西南昌330045

出　　处：《植物分类与资源学报》2014年第4期443-448,共6页Plant Diversity

基　　金：国家自然科学基金(31160043;31160082);国家科技支撑计划(2012BAC11B02)

摘　　要：钩栲（Castanopsis tibetana）是中国亚热带常绿阔叶林具有代表性的建群种之一，由于生境片段化的影响，其遗传多样性和进化潜力受到严重威胁。为研究钩栲遗传多样性及群体遗传特征，本研究从来自中华栲（Castanopsis chinensis）、苦槠栲（C．sclerophylla）、尖叶栲（C．cuspidata）、日本栲（C．sieboldii）4种栲属植物的51个微卫星位点中，筛选出35个在钩栲中具有稳定PCR扩增的位点：采用4个钩栲群体24个个体进行检测，发现11个位点具有较低的多态性，其中等位基因数目（N。）为2。7，平均4．5：观测杂合度（Ho）为0．083～0．917，平均0．505；预期杂合度（HE）为0．236～0．667，平均0．471。研究结果对钩栲遗传多样性研究和保护具有重要作用。Castanopsis tibetana is a typical and dominant component in subtropical evergreen broad-leaved forests of China. However, its genetic diversity and long-term persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, cross-species amplification of 51 microsatellite loci which are de- rived from four congeneric species, i.e., Castanopsis chinensis, C. sclerophflla, C. cuspidata and C. sieboldii, were tested in C. tibetana. Thirty-five loci were successfully amplified in C. tibetana, among them 11 were polymorphic across four wild populations of C. tibetana （24 individuals in total）. Genetic diversity of four C. tibetana populations （Jiangxi） was relatively low. The number of alleles （ Nn ） ranged from 2 to 7 （ average 4. 5 ） and the ranges of observed （ Ho ） and expected （ HE ） heterozygosities were 0. 505 and 0. 471, respectively. The results will facilitate the study and conservation of genetic diversity of C. tibetana.

关 键 词：钩栲 微卫星 通用性 遗传多样性 

分 类 号：S792.17[农业科学—林木遗传育种]