糖耐量减低患者维生素D、维生素D受体基因FokI多态性与胰岛素抵抗的相关性研究  

Association of serum vitamin D levels and vitamin D receptor gene polymorphism with insulin resistance in patients with impaired glucose tolerance

作　　者：侯丽君[1] 唐先格[1] 亓文波[2] 

机构地区：[1]泰山医学院附属医院内分泌科,泰安271000 [2]泰安市中心医院内分泌科

出　　处：《中国糖尿病杂志》2014年第7期586-589,共4页Chinese Journal of Diabetes

摘　　要：目的 探讨IGT者25羟基维生素D3（25-OH-VD3）、维生素D受体（VDR）基因FokI多态性与IR的相关性。 方法 选取IGT者（IGT组）94例及正常对照（NC）组54名，采用PCR-RLFP检测VDR基因FokI多态性，ELISA检测25-OH-VD3，比较各组基因型及等位基因频率。 结果 ⑴与NC组比较，IGT组血清25-OH-VD3较低[（22.05±2.37） vs （32.04± 2.64） ng/ml，P〈0.05];⑵与NC比较，IGT组“f”等位基因频率升高（46.8% vs 25.9%，FF：30.9% vs 57.4%;Ff： 46.9% vs 33.3%;ff：22.3% vs 9.3%，P〈0.05）。与FF基因型比较，Ff/ff基因型患IGT危险增加（OR=2.613，95%CI=1.239～5.512，P=0.018;OR=4.490，95%CI=1.496～13.473，P=0.010）。与F型比较，携带“f”等位基因患IGT风险增加（OR=2.465，95%CI=1.188～5.116，P=0.023）。Ff、ff基因型与FF型比较，HOMA-IR增高（P〈0.05）;VDR基因FokI位点ff基因型者较其它两种基因型IR更明显（P〈0.01）;⑶Logistic回归分析显示，VDR基因FokI ff与IR呈正相关（OR=1.761，95%CI：1.050～2.970，P〈0.05），25-OH-VD3与IR呈负相关（β=-1.187，OR=0.461，95%CI：0.187～0.741，P〈0.05）。 结论 血清25-OH-VD3水平与IR呈负相关，VDR基因FokI可能与IGT者IR有关。Objectives To explore the association of VDR FokI polymorphism and the levels of serum 25-OH-VD3 with insulin resistance （IR） in patients with impaired glucose tolerance （IGT）. Methods 94 subjects with IGT（IGT group） and 54 healthy examinees（NC group） were enrolled. Vitamin D receptor gene FokI polymorphism was detected by PCR-RFLP in all subjects. The levels of 25-hydroxy vitamin D3 were examined by ELISA. The genotype and its frequencies were compared among groups. Results （1） The serum level of 25-OH-VD3 was lower in IGT group than in NC group（22.05±2.37 vs 32.04±2.64, P〈0.05）.（2） The “f” allele frequencies was significant higher in IGT group than in NC Group （f： 46.8% vs 25.9%; FF： 30.9% vs 57.4%; Ff： 46.9% vs 33.3%; ff： 22.3% vs 9.3%）. The patients with Ff or ff genotype had increased risks of IGT than those with FF genotype （OR=2.613,95%CI=1.239～5.512,P=0.018; OR=4.490,95%CI=1.496～13.473,P=0.010）. “f” allele had increased risk of IGT as compared with those carrying “F” allele. HOMA-IR was significantly lower in groups with FF genotype than with Ff or ff genotype. （3） Multivariate logistic regression analysis showed polymorphisms of VDR genes were correlative with insulin resistance（OR=1.761,95%CI：1.050～2.970,P〈0.05）. There was a significantly negative correlation between serum 25（OH）VD3 and HOMA-IR（β=-1.187,OR=0.461,95%CI：0.187～0.741,P〈0.05）. Conclusions Levels of 25-OH-VD3 and insulin resistance were negatively correlated and polymorphisms of VDR genes may be associated with insulin resistance.

关 键 词：糖耐量减低 胰岛素抵抗 维生素D受体基因多态性 

分 类 号：R587.1[医药卫生—内分泌]