循环miRNA-126在2型糖尿病患者中的表达变化及其相关性研究  

The change of expression level of circulating miRNA-126 in patients with type 2 diabetes and its relative factors

作　　者：任彦红[1] 黄婷[1] 时学秀 闫丹丹[1] 栗夏连[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052

出　　处：《中国糖尿病杂志》2014年第7期633-636,共4页Chinese Journal of Diabetes

基　　金：河南省卫生厅省部共建项目(201201015)

摘　　要：目的探讨不同阶段T2DM患者循环miRNA-126水平变化及与内皮型一氧化氮合酶(eNOS)及相关代谢指标的关系。方法采用RT-PCR检测血浆miRNA-126表达水平,ELISA测定eNOS水平,分析miRNA-126与eNOS及相关代谢指标的关系。结果 (1)与健康对照(NGT)组比较,miRNA-126在T2DM前期(Pre-T2DM)组、单纯T2DM(T2DM)组及T2DM合并大血管病变(T2DM+CVD)组中均下降(P<0.05)。(2)miRNA-126与LDL-C、DBP及eNOS呈正相关(r=0.224、0.164、0.661,P均<0.05),与FPG、HDL-C、HbA1c及年龄呈负相关(r=-0.293、-0.153、-0.258、-0.277,P均<0.05)。(3)多元逐步回归分析显示,FPG(β=-0.159,P<0.01)、年龄(β=-0.030,P<0.01)、LDL-C(β=0.208,P=0.037)、eNOS(β=0.353,P<0.01)是循环miRNA-126的影响因素。结论循环miRNA-126在不同阶段T2DM患者中均呈低表达,FPG、年龄、LDL-C及eNOS是影响miRNA-126的相关因素,推测miRNA-126可能受糖脂代谢的调控,其下调可能与血管内皮功能障碍有关。Objective To investigate the changes in the levels of plasma miRNA-126 in different stages of T2DM patients,and the correlation of miRNA-126 with endothelial nitric oxide synthase（ eNOS ）and metabolic parameters. Methods The levels of plasma miRNA-126 were measured by real-time PCR （RT-PCR） . The levels of plasma eNOS were measured by enzyme-linked immunosorbent assay（ELISA）. The correlation of plasma miR-126 with eNOS and metabolic parameters was also analyzed. Results （1） The levels of plasma miRNA–126 in pre-diabetes （A）group, simple T2DM （B）group and T2DM with macroangiopathy （C）group were significantly lower（P〈0.05） compared with healthy control （NC） group. （2） There were positive correlations between miRNA-126 and LDL-C, DBP and eNOS（r=0.224,0.164,0.661, respectively,P〈0.05）. There were negative correlations between miRNA-126 and FPG, HDL-C,HbA1c,and age（r=-0.293,-0.153,-0.258,-0.277, respectively, P〈0.05）. （3） Multiple stepwise regression analysis showed that FPG （β=-0.159,P〈0.01）, age （β=-0.030,P〈0.01）, LDL-C （β=0.208, P = 0.037） and eNOS（β=0.353,P〈0.01）were the correlative factors for plasma miRNA-126 . Conclusion Circulating miRNA-126 expression levels are decreased in ditterent stages ot patients with T2DM. FPG, age, LDL-C and eNOS are the correlative factors for the miRNA-126, suggesting that miRNA-126 may be regulated by blood glucose and lipid metabolism.

关 键 词：糖尿病 2型 miRNA-126 血管病变 糖脂代谢 内皮功能障碍 

分 类 号：R587.1[医药卫生—内分泌]