蓬草除痹汤对类风湿关节炎免疫调节作用的实验研究  

Experimental study on immunomodulatory effects on rheumatoid arthritis by Pengcao rheumatism-syndrome eliminating decoction

作　　者：刘春景[1] 王霞[1] 魏民[1] 优鲁都斯[1] 王学涵[1] 董晶晶[2] 宋继荣[2] 

机构地区：[1]新疆医科大学第五附属医院,新疆乌鲁木齐830011 [2]新疆医科大学,新疆乌鲁木齐830054

出　　处：《现代中西医结合杂志》2014年第22期2399-2401,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2012211A026)

摘　　要：目的观察蓬草除痹汤(PCCB)对Ⅱ型胶原诱导关节炎(CIA)大鼠模型外周血中细胞因子IL-17、IL-1β、TNF-α及T细胞亚群CD4+T、CD8+T、CD4+T/CD8+T的干预作用,探讨PCCB治疗类风湿关节炎(RA)的抗炎免疫作用机制。方法建立CIA大鼠模型,随机分为PCCB组、雷公藤组、空白组及模型组,连续给药28 d,采用酶联免疫吸附(ELISA)法、流式细胞术评价PCCB对CIA大鼠关节炎指数(AI)及大鼠血清IL-17、IL-1β、TNF-α和CD4+T、CD8+T、CD4+T/CD8+T表达的影响。结果治疗28 d后,PCCB组、雷公藤组关节肿胀度及AI显著改善(P均<0.01);血清IL-17、IL-1β、TNF-α水平明显降低(P均<0.01);CD4+T降低,CD8+T升高,CD4+T/CD8+T显著降低,且PCCB组的抑制作用明显优于雷公藤组(P<0.05)。结论 PCCB能有效改善CIA大鼠关节炎指数,下调大鼠血清中致炎因子IL-17、IL-1β、TNF-α的水平,调节大鼠血清中CD4+T、CD8+T及CD4+T/CD8+T比例,这可能是PCCB治疗RA的主要机制,为临床应用及进一步研究奠定了基础。Objective It is to observe the intervention effect on cytokine of IL -17 , IL-1β, TNF-αand T-lymphocyte subset of CD4 ＋T,CD8 ＋T and CD4 ＋T/CD8 ＋T in peripheral blood of rats with collagen -induced arthritis Ⅱ（ CIA） treated by the Peng Cao rheumatism -syndrome eliminating decoction （ PCCB, abbreviation for the decoction＇s Chinese name： Peng Cao Chu Bi ） , and to explore its mechanism of immune anti -inflammatory action .Methods CIA rat models were established and randomly divided them into 4 groups：PCCB group , tripterygium glycosides group , blank group and model group , and all the groups were treated for 28 days.Enzyme-linked immunosorbent assay （ELISA） and flow cytometry were used to evaluate the effects on arthritis index （ AI） and on the expression levels of IL -17 , IL-1βand TNF-αand T-lymphocyte subset of CD4 ＋T,CD8 ＋T and CD4 ＋T/CD8 ＋T in serum of CIA rats treated by PCCB .Results After 28 days＇treatment, compared with model group, the AI of the rats in the PCCB and TG groups were significantly improved （P＆lt;0.01）, the levels of IL-17, IL-1βand TNF-αin serum were reduced （P＆lt;0.01）, CD4 ＋were reduced, CD8 ＋increased （P＆lt;0.01） and CD4 ＋T/CD8 ＋T reduced obviously （P＆lt;0.05）, while the inhibiting effect in PCCB group were superior obviously to that in TG group （P＆lt;0. 05）.Conclusion PCCB could effectively improve the AI of CIA rats , reduce the level of pro-inflammatory cytokine of IL -1, IL-1βand in serum, regulate the ratio of CD4 ＋T,CD8 ＋T and CD4 ＋T/CD8 ＋T in serum of the rats.That might suggest a key mechanism to remedy RA by PCCB , which may lay a foundation of clinical application for RA treatment .

关 键 词：蓬草除痹汤 CIA大鼠 细胞因子 T细胞亚群 

分 类 号：R332[医药卫生—人体生理学]