利用混料设计优化多杀菌素发酵培养基  

Mixture design for optimization of spinosad production

作　　者：阎宝清 夏立秋[1] 龙如花 张由恒 陈宏[1] 

机构地区：[1]湖南师范大学生命科学学院微生物分子生物学国家重点实验室培育基地,湖南长沙410081

出　　处：《食品与机械》2014年第4期200-203,共4页Food and Machinery

基　　金：国家重点基础研究发展计划(编号:2011CB111605);国家自然科学基金(编号:31070006)

摘　　要：利用Plackett-Burman试验设计从8种营养成分中筛选出4种对多杀菌素产量影响较显著的因子:葡萄糖、棉籽粉、玉米浆、豆饼粉,而后通过D-最优混料设计,对这4种成分进行优化设计。以多杀菌素的产量为期望,进行回归分析并建立关于多杀菌素产量的二阶拟合方程,确定4种成分的最佳比例为:葡萄糖59 g/L、棉籽粉28 g/L、玉米浆6.5g/L、豆饼粉6.5g/L,根据方程预测产量理论最高值为256.75mg/L,按此配方进发酵验证,产量为249.9mg/L,证明该方程的拟合性较好。Glucose, cotton seed flour, corn steep liquor and soybean flour were found to be the most important factors affecting spinosad production using Plackett-Burman design from 8 factors. Then, Doptimal mixture design to optimize the 4 factors. Regression model on Spinosad production was established, and a second-order polynomial model which expressed the good relationship of variables and response. The optimum fermentation medium for spinosad production were as follows： Glucose 59 g/L, cotton seed flour 28 g/L, corn steep liquor 6.5 g/L, soybean flour 6.5 g/L, the predicted yield was 256.75 mg/L. The yield of spinosad was 249.9 mg/L using the optimized medium, and this proved the equation fitted the yield of spinosad accurately.

关 键 词：多杀菌素 培养基 Plackett—Burman试验设计 D-最优混料设计 

分 类 号：S482.3[农业科学—农药学]