HPLC法同时测定人参及其制剂中16种人参皂苷  

Simultaneous determination of sixteen ginsenosides in Panax ginseng and its preparation by HPLC

作　　者：郭冲[1] 郜玉钢[1] 臧埔 何忠梅[1] 赵岩[1] 祝洪艳[1] 杨鹤[1] 董微[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《中草药》2014年第14期2009-2013,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划项目(2011BAI03B010602);国家公益性行业科研专项(201303111);国家科技重大专项子课题(2012ZX09304006);吉林省基础研究项目(20130102075JC);吉林省科技厅重点项目(20110228);吉林省科技条件与平台建设计划(20112101);吉林省现代农业产业技术体系建设项目(201218)

摘　　要：目的建立同时测定人参及其制剂中16种人参皂苷的HPLC方法。方法采用C18（150mm×4．6mm，5μm）色谱柱；流动相为乙腈和水，梯度洗脱，体积流量1．0mL／min，检测波长203nm，柱温35℃。结果16种人参皂苷Rgl、Re、Rf、Rbl、Rg2、Rc、Rb2、Rb3、F1、Rd、F2、Rg3、Rh2及原人参三醇、compoundK、原人参二醇均得到良好分离，线性关系良好（r≥0．9990）。加样回收率均在95％-102％，RSD〈2％。结论该方法快捷简便、稳定可靠，可应用于人参及其制剂的质量控制。Objective In order to evaluate the quality of Panax ginseng and its preparation, a simple and accurate HPLC method for determining the contents of 16 ginsenosides from P ginseng was established. Methods The chromatographic separation was achieved on a C18 column （150 mm ×4.6 mm, 5μm） using a mobile phase made up of acetonitrie and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35 ℃, respectively. Results Sixteen ginsenosides （Rgl, Re, Rf, Rbl, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, compound K, Rh2, and protopanaxadiol） were separated at baseline with good linearity （r 〉 0.999 0）. The recovery rates were 95%-102% （RSD 〈 2%）. Conclusion The method is simple, fast, accurate, and could be applied to the quality control of P. ginseng and its preparation.

关 键 词：人参 人参皂苷 HPLC人参皂苷Rgl 人参皂苷Re 人参皂苷Rf 人参皂苷Rb1 人参皂苷RG2 人参皂苷Rc 人参皂苷Rb2 人参皂苷RB3 人参皂苷F1 人参皂苷Rd 人参皂苷F2 人参皂苷Rg3 原人参三醇 compoundK 人参皂苷Rh2 原人参二醇 

分 类 号：R286.02[医药卫生—中药学]