正交试验优化大鼠糖尿病胃轻瘫模型  

Optimization of diabetic gastroparesis rat model by orthogonal design

作　　者：吴国泰[1,2] 杜丽东[1,2] 景琪[1] 高云娟[1] 刘五州[1] 李应东 任远[1,2] 

机构地区：[1]甘肃中医学院药理学教研室,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

出　　处：《中草药》2014年第14期2047-2050,共4页Chinese Traditional and Herbal Drugs

基　　金："十二五"国家科技支撑计划项目子课题(2011BAI05B0206);甘肃省自然科学基金项目(1212RJZA079);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-004)

摘　　要：目的通过正交试验优化糖尿病胃轻瘫大鼠模型的制备方法。方法采用四氧嘧啶(ALX)法复制大鼠糖尿病胃轻瘫模型;采用4因素3水平的正交试验设计,以空腹血糖、胃内残留率和血浆胃动素(MOT)水平综合评分为评价指标,观察不同影响因素对大鼠糖尿病胃轻瘫模型形成的影响。结果饲料类型对大鼠糖尿病胃轻瘫模型的影响不明显,造模时间、ALX剂量对大鼠糖尿病胃轻瘫的形成有决定性影响,3个影响因素重要性依次为造模时间>ALX剂量>饲料类型。结论通过优化试验方法,可有效提高糖尿病胃轻瘫大鼠模型的造模效果,为科学评价糖尿病胃轻瘫的药物研究提供技术平台。Objective To optimizethepreparation methods of diabetic gastroparesis rat model by orthogonal test. Methods The diabetes gastroparesis rat model was duplicated with alloxan （ALX） by using four factors and three levels of orthogonal design. The fasting blood glucose, gastric emptying rate, and plasma motilin level were scored as evaluation indexes, and the influence of different factors on diabetic gastroparesis model rats was observed. Results The effect of feed type on the diabetic gastroparesis rat model was not obvious, but the effects of modeling time and modeling agent ALX dose on diabetic gastroparesis rat model were decisive. The order of the importance was as follows： modeling time 〉 dose ofALX 〉 feed types. Conclusion The optimized test method can effectively improve the diabetic gastroparesis rat model and provide a technical platform for the study on drug evaluation of diabetic gastroparesis.

关 键 词：四氧嘧啶 糖尿病胃轻瘫 正交试验 胃动素 动物模型 

分 类 号：R965.1[医药卫生—药理学]