控制性低压后处理对兔脊髓缺血再灌注损伤的影响及其机制探讨  

Effect of controlled low-pressure perfusion postconditioning on spinal cord ischemia-reperfusion injury in rabbits and the mechanism

作　　者：艾春雨[1] 江晓菁[1] 马虹[1] 王俊科[1] 

机构地区：[1]中国医科大学附属第一医院,沈阳110001

出　　处：《山东医药》2014年第28期7-9,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30740092)

摘　　要：目的：探讨控制性低压后处理对兔脊髓缺血再灌注损伤的影响及机制。方法32只雄性日本大耳白兔随机分为4组，每组8只，分别为假手术对照组（S组）、缺血再灌注损伤组（I/R组）、控制性低压后处理组（P组）、控制性低压后处理＋ERK1/2抑制剂PD98059组（ PD组）。于再灌注后24 h处死动物，取L3～5段脊髓组织标本。TUNEL法检测细胞凋亡；免疫细胞化学SP法检测脊髓组织p-ERK1/2、Caspase-3；羟胺法测定脊髓组织超氧化物歧化酶（SOD），硫代巴比妥酸法测定脊髓组织丙二醛（MDA）。结果与I/R组比较，P组凋亡指数降低，脊髓组织MDA降低、SOD增加、Caspase-3减少、p-ERK1/2增加，P均＜0．05；与P组比较，PD组凋亡指数升高，脊髓组织MDA增加、SOD减少、Caspase-3增加、p-ERK1/2减少，P均＜0．05。结论控制性低压后处理对脊髓缺血再灌注损伤具有保护作用，其机制可能与活化ERK1/2、抑制Caspase-3有关。Objective To investigate the effect of controlled low-pressure perfusion postconditioning on the spinal cord ischemia-reperfusion injury in rabbits and the mechanism .Methods Thirty-two Japanese white rabbits were randomly di-vided into four groups （n=8）： the sham group （group S）, ischemia-reperfusion （I/R） group, controlled low-pressure perfusion postconditioning group （group P）, controlled low-pressure perfusion postconditioning ＋PD98059 （PD） group. All rabbits were sacrificed 24 h after reperfusion , L3-5 segments of the spinal cord were removed .TUNEL method was used to detect the apoptosis , the expression of p-ERK 1/2 and caspase-3 in the spinal cord tissues was detected by immunohisto-chemical SP method , the superoxide dismutase （ SOD） was determined by hydroxylamine method , and the malonyldialde-hyde （ MDA） in the spinal cord tissues was detected by thiobarbituric acid method .Results Compared with I/R group, the apoptosis index decreased, and in the spinal cord tissues, MDA decreased, SOD increased, caspase-3 decreased, p-ERK1/2 increased in the group P （all P〈0.05）;compared with group P, the apoptosis index increased , and in the spinal cord tissues, MDA increased, SOD decreased, caspase-3 increased, p-ERK1/2 decreased in the PD group （ all P 〈0.05）.Conclusions Controlled low-pressure perfusion postconditioning has the protective effect on the spinal cord I /R injury, whose mechanism may be related to activation of ERK 1/2 pathway and inhibition of the expression of caspase-3.

关 键 词：缺血再灌注损伤 控制性低压后处理 脊髓 兔 

分 类 号：R654.2[医药卫生—外科学]