橡胶树产排胶相关基因在死皮和健康橡胶树中表达模式分析  

Expression Patterns of Rubber Biosynthesis and Flow-related Genes Between Healthy and Tapping Panel Dryness Rubber Trees

作　　者：李德军[1] 邓治[1] 刘向红[1,2] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]海南大学农学院,海南海口570228

出　　处：《热带作物学报》2014年第7期1336-1340,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金橡胶树死皮发生关键基因-翻译控制肿瘤蛋白特性及功能研究(No.31270651);橡胶树排胶相关ADF基因参与乳管堵塞的机制研究(No.31200514);利用分子生物学方法研究橡胶树死皮发生分子机制(No.30960310)

摘　　要：橡胶树死皮(Tapping Panel Dryness,TPD)是天然橡胶单产提高的主要限制因子之一,给中国橡胶种植业带来巨大经济损失。本文利用RT-PCR技术分析14个产排胶相关基因在死皮和健康橡胶树树皮中表达模式。研究结果表明,有8个基因在健康橡胶树树皮中上调表达,其分别是法尼基焦磷酸合酶、小橡胶粒子蛋白、橡胶素、蔗糖转运蛋白5、橡胶延伸因子、几丁质酶、HMG辅酶A还原酶和HMG辅酶A合成酶,有4个基因在死皮橡胶树树皮中上调表达,他们分别是橡胶转移酶、牻牛儿基牻牛儿基二磷酸合酶、蔗糖转运蛋白2a和β-1,3-葡聚糖酶;另外,蔗糖转运蛋白2b和蔗糖转运蛋白1表达模式没有发生变化。此结果为深入揭示橡胶树死皮发生分子机制提供新观点。Tapping panel dryness （TPD） is one of key limiting factors for increasing the yield of natural rubber,and it causes serious economic loss to the rubber production in China.In this study,the expression profiles of fourteen genes related to rubber biosynthesis and flow were analyzed between healthy and TPD rubber tree barks.Eight genes,such as farnesyl-diphosphate synthase,small rubber particle protein,hevein,sucrose transporter 5,rubber elongation factor,chitinase,HMG-CoA reductase and HMG-CoA synthase,were up-regulated in barks of healthy rubber tree; four genes including cis-Prenyl transferase,geranylgeranyl diphosphate synthase,sucrose transporter 2a and β-1,3-glucanase were up-regulated in barks of TPD rubber tree; In addition,the expression patterns of sucrose transporter 2b and sucrose transporter 1 were not changed between healthy and TPD rubber trees.The results provided new insights into the molecular mechanism underlying TPD in rubber tree.

关 键 词：橡胶树 死皮 产排胶相关基因 

分 类 号：S794.1[农业科学—林木遗传育种]