铍针术对神经末梢张力性疼痛模型家兔局部5-羟色胺含量的影响  

Effect of Pi-Zhen on 5-HT Content in Nerve Endings Tension Pain Rabbit Model

作　　者：苏永强[1] 董福慧[1] 王德龙[1] 王萱[1] 

机构地区：[1]中国中医科学院望京医院,北京100102

出　　处：《世界科学技术-中医药现代化》2014年第6期1256-1260,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(30973817):神经末梢张力性疼痛的治疗机理研究;负责人:董福慧

摘　　要：目的:探讨铍针对神经末梢张力性疼痛模型家兔5-羟色胺(5-HT)的影响。方法:实验用健康3月龄雄性大耳白兔42只,按照完全随机方法分为正常组、模型组、药物组、毫针组、铍针组,制作家兔神经末梢张力性疼痛模型,各组的家兔均需要在第5、10、20、30、40、50、60 min时使用1 mL的注射器向造模部位的浅、深筋膜之间注入1 mL生理盐水进行冲洗并抽出溶液,ELISA免疫学检测方法对提取液中的5-HT进行检测。结果:铍针干预能够降低5-HT含量,随着时间延长5-HT含量逐渐减少,与模型组相比,治疗组5-HT含量在不同时间点均显著降低(P<0.01);甚至铍针组5 min时显著高于正常组(P<0.01);与药物组相比,5、10、20、30、40 min时毫针组、铍针组5-HT含量显著升高(P<0.01);与正常组相比,铍针组在40、50、60 min时5-HT含量明显降低(P<0.05);与毫针组相比,20、30、40、50、60 min时5-HT含量显著降低(P<0.01)。其余时间点组间比较无明显差异。结论:铍针可以通过减低局部软组织张力、减少了机体内5-HT的释放并促进其降解等方面发挥镇痛作用。This article was aimed to explore theeffect of Pi-Zhen（PZ） on 5-hydroxytryptamine（5-HT） in nerve end-ings tension painrabbitmodel. Forty-two healthy male big ear whiterabbits of three months old were randomly divided into 5 groups, which were the normal group,model group, drug group, Hao-Zhen （HZ） group, and PZ group. Nerve endings tension pain modelswere established. No operation was made in the normal group. The amount of 1 ml saline was injected to the operative sitebetween the shallow and deep fascia in each rabbit and then draw out at 5, 10, 20, 30, 40, 50, 60 min after operation. The 5-HT level in extracting solution of different groups was determined with＆nbsp;ELISA method. The results showed that PZ interventioncan reducethe 5-HT level ofextracting solution in operative site; and with the increasing of time, the level of 5-HT was gradually reduced. Compared with the model group, the contents of 5-HT in the treatment group were significantly decreased at different time points（P〈0.01）. The content of 5-HT in the PZ group was significantly higher than that of the normal group at 5 min （P〈0.01）. Compared with the drug group, the contents of 5-HT in the HZ group and PZ group were significantly increased at 5, 10, 20, 30, 40 min （P〈0.01）. Compared with the normal group, the contents of 5-HT in the PZ group was significantly decreased at 40, 50, 60 min （P〈0.05）. Compared with the HZ group, the contents of 5-HT were significantly decreased at 20, 30, 40, 50, 60 min （P〈0.01）. There was no difference at other time points. It was concluded that PZ can ease pain through reducing the tension of local soft tissues, decreasing 5-HT releasing, and promoting its degradation.

关 键 词：铍针 神经末梢张力性疼痛 5-羟色胺 局部软组织张力 

分 类 号：R246[医药卫生—针灸推拿学]