齐墩果酸抑制3T3-L1脂肪细胞脂质堆积  

Inhibitory Effect of Oleanolic Acid on Lipid Accumulation in Cytoplasm of 3T3-L1 Adipocyte

作　　者：王石峰[1] 翟晨曦[1] 刘青[1] 张燕玲[1] 栗世铀[2] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国科学院北京基因组研究所,北京100101

出　　处：《世界科学技术-中医药现代化》2014年第6期1353-1358,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家科技支撑计划项目(2008BAI51B01):中药有效成分群辨识技术研究;负责人:张宏桂;北京中医药大学开放课题(2013-SYSK-FKT-04):中药基础与新药研究重点实验室;负责人:乔延江

摘　　要：目的:从57个中药化合物筛选具有抑制3T3-L1脂肪细胞脂质堆积的活性成分。方法:3T3-L1细胞培养至融合后用化合物进行干预,并诱导使其分化,采用高内涵影像方法检测细胞中脂滴含量。对发现的活性成分用ToxInsight体外毒性检测方法评价其肝毒性。结果:发现并验证活性成分齐墩果酸(OA)具有显著的脂质形成抑制作用,其半数抑制浓度(IC50)为14.5μmol·L-1,且在60μmol·L-1测试浓度范围内对HepG2细胞无显著损伤作用。结论:OA具有显著的降低脂质形成作用,是潜在的降脂候选化合物。A collection of 57 natural compounds derived from Chinese herbal medicines were evaluated for their an-ti-adipogenic effects. The lipid droplets in differentiated 3T3-L1 adipocytes treated with tested substrate were de-tected by an image-based assay. The results demonstrated that oleanolic acid （OA） inhibited lipid droplets accumula-tion in 3T3-L1 adipocytes in a dose-dependent manner with the IC50 value of 14.5 μmol·L-1. In addition, ToxInsight assay showed that OA was free of liver injury for HepG2 cells within 60 μmol·L-1. It was concluded that OA, which had an obvious anti-adipogenic effect, can be a candidate for hyperlipidemia therapy.

关 键 词：齐墩果酸 3T3-L1脂肪细胞 脂质形成 

分 类 号：R285.5[医药卫生—中药学]