穿山龙水溶性总皂苷对RA细胞模型及其下游因子的影响  

Inhibition Effect of Water-solubility Nipponica Saponin on NF-κB Pathway of Rheumatoid Cellular Model

作　　者：段一娜[1] 王明娟[1] 杨佳琪[1] 高玉峰[1] 程广民 米术斌[1] 宋鸿儒[1] 

机构地区：[1]承德医学院基础医学院,承德067000 [2]隆化县唐三营卫生院,隆化068150

出　　处：《世界科学技术-中医药现代化》2014年第6期1396-1400,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：河北省教育厅指令课题(2011163):薯蓣皂苷对IL-17和TNF-α诱导大鼠滑膜细胞株NF-κB通路的影响;负责人:段一娜;承德市科技局指导课题(201121024):薯蓣皂苷片对类风湿关节炎的应用研究;负责人:段一娜;河北省教育厅高等学校科学技术项目(ZH200806):穿山龙总皂苷对CIA大鼠化膜细胞炎性因子网络及NF-κB活性调控机制的研究;负责人:宋鸿儒;国家自然科学基金委重点项目(30873420):穿山龙总皂苷对CIA大鼠血管新生相关细胞因子的影响及其调控机制的研究;负责人:宋鸿儒

摘　　要：目的:观察穿山龙水溶性总皂苷对TNF-α加IL-17诱导的大鼠成纤维样滑膜细胞株RSC-364核转录因子NF-κB活化的调控作用及下游基因TNF-α、IL-1、ICAM-1、MMP-2、MMP-3分泌的影响。方法:用IL-17加TNF-α刺激RSC-364建立类风湿性关节炎(RA)细胞模型,以不同浓度穿山龙水溶性总皂苷干预,采用半定量RT-PCR的方法检测各组NF-κBp65基因和IκB-α基因的表达,酶联免疫吸附试验(ELISA)检测细胞培养液上清中TNF-α、IL-1、ICAM-1、MMP-2、MMP-3蛋白水平的变化。结果:穿山龙水溶性总皂苷可呈浓度依赖性抑制TNF-α加IL-17刺激的RSC-364中NF-κBp65的活化,提升IκB-α的表达,并抑制TNF-α、IL-1、ICAM-1、MMP-2、MMP-3的分泌。结论:穿山龙水溶性总皂苷可抑制NF-κB通路的活化,阻碍下游多种基因的分泌与活化,提示其可能具有抑制RA滑膜炎症的作用。This study was aimed to observe the influence of water-solubility nipponica saponin on activation of TNF-α＋IL-17-induced rat fibroblast-like synovial cell line RSC-364 cellular model nuclear transcription factor NF-κB pathway as well as TNF-α, IL-1, ICAM-1, MMP-2, MMP-3 secretion. IL-17＋ TNF-α were used for stimulating RSC-364 to establish rheumatoid arthritis （RA） cellular model. Water-solubility nipponica saponin in different con-centrations was used for intervention. The influence of water-solubility nipponica saponin in different concentrations on cell viability was detected by semi-quantitative RT-PCR method. Changes in the level of TNF-α, IL-1, ICAM-1, MMP-2, and MMP-3 of culture supernatant were detected by ELISA. The results showed that the activation of NF-κB p65 in RSC-364 stimulated by TNF-α＋ IL-17 can be inhibited by water-solubility nipponica saponin ac-cording to its concentration. It improved IκB-α expression, and inhibited TNF-α, IL-1, ICAM-1, MMP-2 and MMP-3 secretion. It was concluded that water-solubility nipponica saponin can inhibit the activation of NF-κB pathway, hinder the secretion and activation of multiple downstream genes, which may be its effect in inhibiting syn-ovial inflammation in RA.

关 键 词：类风湿性关节炎 穿山龙水溶性总皂苷 大鼠成纤维样滑膜细胞株 NF-ΚBP65 

分 类 号：R285.5[医药卫生—中药学]