内生链霉菌Streptomyces sp.LC18中安莎霉素类化合物的发现  

Ansamycins Produced by Endophytic Streptomyces sp.LC18

作　　者：李善仁[1] 孟瑾[2] 王浩鑫[3] 鲁春华[2] 沈月毛[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361102 [2]山东大学药学院,山东济南250012 [3]山东大学生命科学学院,山东济南250100

出　　处：《厦门大学学报（自然科学版）》2014年第4期588-592,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(31100079);山东大学自主创新基金(2010TB016;2010TS072)

摘　　要：以一株红树林植物内生链霉菌Streptomyces sp.LC18为研究对象,通过固体发酵培养分离安莎霉素类代谢产物.采用凝胶柱层析和硅胶柱层析等对化学成分进行分离纯化,通过核磁共振和高分辨质谱对化学结构进行鉴定,初步研究了化合物抗菌和细胞毒活性.从10L固体发酵提取物中分离得到3个安莎霉素类化合物,分别鉴定为herbimycin A(1),herbimycin C(2),hygrocin A derivative 6(3),其中化合物1和3对人乳腺癌MDA-MB-231细胞和前列腺癌PC3细胞IC50值均小于5μmol/L.化合物1和3分别属于8酮苯安莎和9酮萘安莎,是从植物内生菌中分离得到2种类型的安莎霉素.Three ansamycin compounds ： herbimycin A （1）, herbimycin C （2）, and hygrocin A derivative 6 （3） were found in the fermentation broth of endophytic Streptomyces sp. LC18 isolated from mangrove tree Kandelia obovata. Their structures were elucidated on the basis of spectroscopic analyses and ESI mass spectrometry,and the antibacterial and antitumor activities of these compounds were evaluated as well.Our results showed that compound 1 and 3 exhibited significant cytotoxicities against the human cancer cell lines MDA-MB-231 and PC3 with ICso less than 5 μmol/L.Moreover,compound 1 and 3 belong to two types of ansamy- cins from the endophytic, i.e. octaketides （benzene） and nonaketides （naphthalene）,respectively.

关 键 词：内生链霉菌 安莎霉素 herbimycin hygrocin A DERIVATIVE 

分 类 号：Q356.1[生物学—遗传学]