粗毛栓菌漆酶基因的克隆、序列分析及荧光定量PCR分析  

Cloning,sequencing and real-time quantitative PCR analysis of laccase gene fromTrametes gallica

作　　者：陈月红[1] 曹庆华[1] 邵欢欢[1] 张昆[1] 谭雪梅[1] 张义正[1] 

机构地区：[1]四川大学生命科学学院、四川省分子生物学和生物技术重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2014年第4期835-841,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30470984)

摘　　要：本研究通过提取不同培养条件下的粗毛栓菌总RNA,利用RT-PCR的方法克隆到1个新的漆酶基因,命名为Tglac3,编码区长1,560bp,预测编码519个氨基酸残基,其分子量为56.6kDa,理论等电点为5.77.用荧光定量RT-PCR法分析了该漆酶基因在不同培养条件下的表达水平,结果显示,高浓度的碳源和氮源均能诱导Tglac3基因的表达.Total RNA from four liquid medium was extracted, and a new laccace gene from Trametes gallica was cloned by RT-PCR, named Tglac3, which contained an ORF of 1,560 bp encoding 519 ami-no acids with a molecular mass of 56.6 kDa and pI 5.77. The transcription level of the laccase gene under different culture conditions was investigated by real-time quantitative PCR. The result showed thathigh concentrations of carbon and nitrogen both can induce the expression of Tglac3 gene.

关 键 词：粗毛栓菌 漆酶 基因克隆 荧光定量PCR 

分 类 号：Q78[生物学—分子生物学]