肝癌细胞株HepG2与正常肝细胞株7702差异微小RNA筛选  

MicroRNA differential screening profile in hepatoma cell line HepG2 and normal hepatocellular cell line 7702

作　　者：李娟[1,2] 刘辉 刘雅歆[3] 叶建蔚 赵军 王建华 温浩 吕国栋 

机构地区：[1]新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地 [2]新疆医科大学基础医学院,乌鲁木齐830011 [3]山东省潍坊市中医院,山东潍坊261000

出　　处：《新疆医科大学学报》2014年第9期1135-1139,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81360251);新疆重大疾病医学重点实验室开放课题资助项目(SKLIB-XJMDR-2012-2);教育部长江学者和创新团队发展计划(IRT1181)

摘　　要：目的初步探讨肝癌细胞株HepG2与正常肝细胞株7702微小RNA(miRNA)分子表达谱的差异。方法选取肝癌细胞株HepG2与正常肝细胞株7702样本分别作为实验组和对照组,采用高通量的miRNA微阵列芯片技术筛选两者间差异表达的miRNA,并运用实时荧光定量聚合酶链反应(RT-qPCR)验证芯片结果的可靠性(样品间变化标准以上调或者下调2倍作为判定的阈值范围)。结果在肝癌细胞株HepG2中,上调2倍的差异表达miRNA分子有32个,其中上调超过8倍差异表达miRNA有12个;下调2倍的差异表达miRNA有26个,其中下调超过8倍的miRNA分子有4个(P<0.05),miRNA上调与下调表达率差异有统计学意义。结论肝癌细胞株HepG2与正常肝细胞株7702的差异表达miRNA分子可能与肝细胞癌(hepatocellular carcinoma,HCC)的发生、转移以及侵袭能力相关,为进一步研究与肿瘤相关的miRNA在肝细胞癌发病机制中的作用奠定基础。Objective To Preliminary explore miRNA differential expression profile between hepatoma cell line HepG2 and normal hepatic cell line 7702.Methods Samples from HepG2 cell line and samples from normal Hepatic Cell line were chosen as the experimental group and the control group,respectively.Endogenous miRNAs were determined by high-throughput miRNA microarray.The expression of selected mature miRNA was also assessed using real-time PCR （The threshold value used to screen up-and down-regulated miRNAs was fold chang 〉 2 ）.Results Twelve microRNAs were up-regulated in the experimental group and were more than 8 times higher than in the control group .Four microRNAs were down-regulated in the experimental group and were more than 8 times higher than in the control group （P〈0.05）.Conclusion These miRNAs with differential expression might be associated with the occurrence, metastasis and invasion ability of hepatocellular carcinoma.It will lay foundation for the further research of＆amp;nbsp;miRNAs associated with cancer role in the pathogenesis of hepatocellular carcinoma （HCC）.

关 键 词：肝细胞癌 微小RNA miRNA微阵列芯片 

分 类 号：Q74[生物学—分子生物学]