香豆素-亚胺型Cu^(2+)识别荧光探针的合成及性能  

Synthesis and properties of Cu^(2+) fluorescence probe based on coumarin-imine

作　　者：王建红[1] 郭心灵[1] 赵会君[1] 罗朝阳[1] 侯旭锋[1,2] 赵瑾[1] 

机构地区：[1]河南大学天然药物与免疫工程重点实验室,河南开封475004 [2]许昌学院化学工程学院,河南许昌461000

出　　处：《化学研究》2014年第4期345-348,358,共5页Chemical Research

基　　金：国家自然科学基金(21272056);河南省科技厅基础与前沿项目(112300413224);河南省教育厅自然科学项目(2010B350002)

摘　　要：以2,4-二羟基水杨醛为原料经Knoevenagel缩合反应、脱乙酰化反应,再与4-二乙氨基水杨醛经缩合反应,进而通过甲基化合成了新型的香豆素荧光探针.利用核磁共振氢谱(1 H NMR)、碳谱(13 C NMR)和高分辩质谱对所合成的目标化合物进行了结构确认,并测定了其荧光光谱.结果表明,目标化合物对Cu2+有较好的选择性和灵敏度,且当Cu2+浓度在0.66~10μmol·L-1范围内时探针的荧光强度与其浓度有较好的线性关系.Job分析法表明探针与金属离子作用形成1∶1结合的络合物.A novel coumarin-based fluorescent probe was synthesized through Knoevenagel condensation,deacelation reaction,condensation with 4-diethylamino salicylaldehyde and methylation with 2,4-dihydroxy salicylaldehyde as the starting material.The structure of the target compound was confirmed by nuclear magnetic resonance spectrometry（^1 H NMR,^13C NMR）and high resolution mass spectrometry,and its fluorescence spectrum was measured.Results demonstrate that the probe exhibits high selectivity and sensitivity towards Cu^2＋.There exists agood linear correlation between the fluorescence intensity of probe and Cu^2＋concentration in the range of 0.66-10μmol·L^-1.In addition,the Job′s plot with fluorescence titration further confirms that the probe interacts with Cu^2＋via1∶1binding forming a complex.

关 键 词：香豆素 铜离子 荧光探针 合成 性能 

分 类 号：O656.2[理学—分析化学]