进境种苗中朱顶红短体线虫的鉴定  

Identification of Pratylenchus hippeastri from imported seedlings

作　　者：顾建锋[1] 王宁[2] 何洁[1] 陈吴健 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012 [2]南京农业大学 [3]浙江出入境检验检疫局

出　　处：《植物检疫》2014年第4期41-46,共6页Plant Quarantine

基　　金：国家科技支撑计划(2012BAK11B03);质检总局科研项目(2013IK279)

摘　　要：2013年,浙江和宁波口岸入境的日本鸡爪槭(Acer palmatum)和以色列孤挺花种球(Hippeastrum vittatum)中分离到一种短体线虫,通过形态学特征以及基于核糖体rRNA-ITS区及28S rRNA-D2/D3区序列的系统发育分析,证实其为朱顶红短体线虫(Pratylenchus hippeastri Inserra,Troccoli,Gozel,Bernard,Dunn&Duncan,2007)。该线虫的主要形态鉴别特征为:虫体细长;头前端平;唇环2个;口针基部球椭圆形;受精囊小,矩形,内无精子;尾圆锥形,末端钝尖,有时腹面有凹陷,或呈光滑的半球形。该线虫至今仅报道发生于美国,这是国际上首次在日本和以色列种苗中截获。Pratylenchus hippeastri was detected from Japan Acer palmatum plant and Israel Hippeastrum vittatum bulbs. It is characterised by slender body, flat and smooth face, head with two lip annuli, ellipsoidal stylet knobs, small rectangular empty spermatheca with large round cavity, and conoid tail with bluntly pointed terminus, usually showing a ventral constriction, or subhemispherical and smooth. It is identified by morphological and DNA sequence analysis of the ITS region and 28S rRNA-D2/D3 gene. This species has only been reported in USA, and this is the first report of its distribution in Japan and Israel.

关 键 词：朱顶红短体线虫 鸡爪槭 孤挺花 ITS D2D3 系统发育 鉴定 

分 类 号：S41-32[农业科学—植物保护]