磺胺间甲氧嘧啶胁迫对小麦幼苗生理生化指标的影响  

Physiological and Biochemical Changes in Wheat Seedlings Under Sulfamonomethoxine Stress

作　　者：金彩霞[1] 郭桦[1] 刘军军[1] 

机构地区：[1]河南师范大学环境学院,河南省环境污染控制重点实验室,黄淮水环境与污染防治省部共建教育部重点实验室,河南新乡452007

出　　处：《农业环境科学学报》2014年第4期634-639,共6页Journal of Agro-Environment Science

基　　金：国家自然科学基金(21107023);中国博士后科学基金项目(20110491001)

摘　　要：采用室内盆栽法,研究了不同浓度磺胺间甲氧嘧啶(SMM)胁迫对小麦幼苗生化指标的影响,初步阐明了SMM对小麦幼苗的毒害机理。研究表明,在实验前3 d,不同浓度胁迫下,各处理叶绿素(Chl)含量之间差异不显著,在实验第5～7 d,高浓度(10 mg·kg-1和20 mg·kg-1)处理Chl含量显著低于对照。实验期间,随SMM浓度增加可溶性蛋白(SP)含量明显下降,SP可作为SMM污染胁迫的生物标记物。SMM胁迫促使丙二醛(MDA)含量相对对照有所增加,且对根系影响强于叶片。SMM胁迫对过氧化物酶(POD)活性整体上表现为激活作用,呈先下降后上升趋势,且根系POD活性远高于叶片。超氧化物歧化酶(SOD)活性在胁迫下表现出类似规律,但随胁迫时间延长,各处理与对照间的差异逐渐减小。Sulfamonomethoxine,widely used in livestock production,may eventually affect plants after entering the soil environment via animal feces.A pot experiment was carried out to investigate the physiological and biochemical responses of wheat seedlings to different con-centrations of sulfamonomethoxine（SMM）.Compared to the control,SMM treatments had no significant influences on chlorophyll（Chl）contents during the first 3 days of the stress,but the treatments with 10 mg·kg^-1and 20 mg·kg^-1reduced the Chl contents in the 5th to 7th day.Addition of SMM markedly decreased the contents of soluble protein（SP）,but increased methyl di aldehyde（MDA）contents as compared to the control,with stronger influence on root system than blades.Peroxidase（POD）activity was decreased by SMM at lower than 10mg·kg^-1,while increased at higher concentrations.Superoxide dismutase（SOD）activity showed similar patterns to POD under SMM stresses,but the differences from the control became less as the stress time lengthened.

关 键 词：磺胺间甲氧嘧啶 小麦 幼苗 生化指标 

分 类 号：X503.231[环境科学与工程—环境工程]