3,4-二羟基甲亚胺-H流动注射法检测食品中硼砂  

Determination of borax in foods by 3,4-dihydroxyazomethine-H flow injection spectrophotometry

作　　者：肖凯[1] 俞凌云[2] 张新申[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]四川出入境检验检疫局技术中心,四川成都610041

出　　处：《中国测试》2014年第2期67-69,共3页China Measurement & Test

摘　　要：基于在乙酸铵缓冲液中3,4-二羟基甲亚胺-H与硼发生显色反应,建立硼的流动注射分光光度自动分析方法.优化试验表明,检测波长430 nm,进样体积500 μL,CTMAB对显色反应具有增敏作用.在确定的条件下,方法的线性范围50.0～1 000.0 μg·L-1,相对标准偏差（RSD） 1.82％,检出限26.0μg·L-1.实测食品样品中的硼,加标回收率97.5％～104.8％.The paper proposes a flow injection spectrophotometric method for determining borax in foods. The method is based on the chromogenic reactions of boron and 3,4-dihydroxyazomethine- H within the buffer solution of ammonium acetate. Chemical factors and FIA variables affecting the system are discussed. The experiment shows the optimal conditions are that the detected wavelength is 430 nm, sample volume is 500 μL, and CTMAB has sensitizing effect. Under the optimal conditions, the linear range of this method is 50.0-1 000.0μg·L-1, the detection limit is 26.0 μg·L-1, and the relative standard deviation （RSD） is 1.82%. When the proposed method was applied to determine borax in food, the recoveries were between 97.5% and 104.8%.

关 键 词：硼砂 3 4-二羟基甲亚胺-H 流动注射 分光光度法 

分 类 号：TQ171.415[化学工程—玻璃工业] O657.32[化学工程—硅酸盐工业]