HPLC法研究SQ0801023在大鼠体内的药动学  

Pharmacokinetics of SQ0801023 in rats by HPLC

作　　者：陈明[1] 侯媛媛[2] 隋婷婷[2] 沈杞容[3] 霍冀川[1] 孙璐[2] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第7期563-567,574,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家重大科技专项基金资助项目(2012ZX09103101-060)

摘　　要：目的建立测定大鼠血浆中SQ0801023的高效液相色谱法,并研究其在大鼠体内的药动学特征。方法 Sprague-Dawley大鼠尾静脉注射SQ0801023（30 mg·kg-1）后,于不同时间点采血,HPLC法测定血浆中SQ0801023的浓度,并计算药动学参数。结果大鼠血浆中的SQ0801023在0.2~50.0 mg·L-1内线性关系良好（r〉0.9991）,定量下限为0.2 mg·L-1,提取回收率为75.2%~87.2%,日内和日间精密度均小于13.7%。SQ0801023在大鼠体内的主要药动学参数：AUC0-t为（3.66±0.70）mg·h·L-1,AUC0-∞为（3.78±0.70）mg·h·L-1,t1/2为（0.22±0.10）h,ρmax为（17.3±3.90）mg·L-1。结论静注SQ0801023后,SQ0801023在大鼠体内快速代谢和消除。Objective To develop a sensitive HPLC method for determination of SQ0801023 in rat plasma and to study the pharmacokinetics in rat after intravenous administration.Methods Blood samples of rats were collected at predetermined intervals for assay of SQ0801023 after intravenous administration of SQ0801023 at dose of 30 mg·kg-1.The plasma concentrations of SQ0801023 were determined by HPLC method and the pharmacokinetic parameters were calculated.Results The good linear range of SQ0801023were from 0.2-50.0 mg·L-1（r ＆gt;0.999 1）.The lower limit of quantification（LLOQ） of the method was0.2 mg·L-1.The extraction recoveries of SQ0801023 from rat plasma were from 75.2%-87.2%,the intra-and inter-assay precisions were below 13.7%,The main pharmacokinetic parameters of rat were as follows：AUC0-twas（3.66 ± 0.70） mg·h·L-1,AUC0-∞was（3.78 ± 0.70） mg·h·L-1,t1/2was（0.22 ±0.10） h,ρmaxwas（17.3 ± 3.90） mg·L-1.Conclusions This assay method is simple,precise and sensitive enough for intravenous pharmacokinetic study of SQ0801023.The SQ0801023 is absorbed and eliminated quickly.

关 键 词：高效液相色谱法 SQ0801023 药动学 

分 类 号：R917[医药卫生—药物分析学]